caa a22 4500 463157249 CHVBK 20180406164738.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s00217-006-0392-z doi (NATIONALLICENCE)springer-10.1007/s00217-006-0392-z A new strategy for the analysis of tetracycline residues in foodstuffs by a surface plasmon resonance biosensor [Elektronische Daten] [Nadine Moeller, Erika Mueller-Seitz, Oliver Scholz, Wolfgang Hillen, Aldert A. Bergwerff, Michael Petz] A new strategy was developed for the establishment of an indirect tetracycline assay using surface plasmon resonance (SPR). The principle for the assay is based on the most important resistance mechanism against tetracycline in gram-negative bacteria. Tetracyclines release the 46.6kDa Tet repressor protein (TetR) from the tet operator (tetO), a biotinylated short double strand DNA sequence bound to a streptavidin biosensor chip. Tetracyclines present in a sample solution bind to the repressor protein, inducing a conformational change accompanied with a reduction of the affinity constant of TetR to tetO. We were able to quantify tetracycline residues in spiked raw milk samples in concentrations corresponding to the maximum residue limit (MRL) set by the European Union. Honey samples could also be analysed but with lower sensitivity. The assay detects seven of the most commonly used tetracyclines in veterinary medicine. Nine antibiotics of five other antibiotic classes were tested and no interference with the SPR assay was found. Thus, the described principle forms the basis for screening assays to routinely test for tetracycline residues in foodstuffs. Springer-Verlag, 2006 Surface plasmon resonance nationallicence Tet repressor nationallicence Tet operator nationallicence Tetracycline nationallicence Milk nationallicence Honey nationallicence Biosensor nationallicence Moeller Nadine Department of Food Chemistry, University of Wuppertal, Gauss-Str. 20, D-42119, Wuppertal, Germany aut Mueller-Seitz Erika Department of Food Chemistry, University of Wuppertal, Gauss-Str. 20, D-42119, Wuppertal, Germany aut Scholz Oliver Department of Microbiology, Biochemistry and Genetics, University of Erlangen-Nuernberg, Staudtstrasse 5, D-91058, Erlangen, Germany aut Hillen Wolfgang Department of Microbiology, Biochemistry and Genetics, University of Erlangen-Nuernberg, Staudtstrasse 5, D-91058, Erlangen, Germany aut Bergwerff Aldert A. Division of Public Health and Food Safety, Institute for Risk Assessment Sciences, Utrecht University, P.O. Box 80.175, NL-3508 TD, Utrecht, The Netherlands aut Petz Michael Department of Food Chemistry, University of Wuppertal, Gauss-Str. 20, D-42119, Wuppertal, Germany aut European Food Research and Technology Springer-Verlag 224/3(2007-01-01), 285-292 1438-2377 224:3<285 2007 224 217 https://doi.org/10.1007/s00217-006-0392-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0392-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moeller Nadine Department of Food Chemistry, University of Wuppertal, Gauss-Str. 20, D-42119, Wuppertal, Germany aut NATIONALLICENCE 700 1- Mueller-Seitz Erika Department of Food Chemistry, University of Wuppertal, Gauss-Str. 20, D-42119, Wuppertal, Germany aut NATIONALLICENCE 700 1- Scholz Oliver Department of Microbiology, Biochemistry and Genetics, University of Erlangen-Nuernberg, Staudtstrasse 5, D-91058, Erlangen, Germany aut NATIONALLICENCE 700 1- Hillen Wolfgang Department of Microbiology, Biochemistry and Genetics, University of Erlangen-Nuernberg, Staudtstrasse 5, D-91058, Erlangen, Germany aut NATIONALLICENCE 700 1- Bergwerff Aldert A. Division of Public Health and Food Safety, Institute for Risk Assessment Sciences, Utrecht University, P.O. Box 80.175, NL-3508 TD, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Petz Michael Department of Food Chemistry, University of Wuppertal, Gauss-Str. 20, D-42119, Wuppertal, Germany aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 224/3(2007-01-01), 285-292 1438-2377 224:3<285 2007 224 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer