caa a22 4500 463157427 CHVBK 20180406164738.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s00217-006-0340-y doi (NATIONALLICENCE)springer-10.1007/s00217-006-0340-y Detection of DNA in virgin olive oils extracted from destoned fruits [Elektronische Daten] [Innocenzo Muzzalupo, Massimiliano Pellegrino, Enzo Perri] Characterization of genetic identity using DNA extracted from olive oil has the potential to facilitate assessment of origin and varietal conformity. Such a prospect is particularly interesting in light of the increased regional spread of olive cultivars and their various contributions to olive oil mixtures for certification of denomination of origin. Towards this goal, we have devised a reliable method for extracting DNA from virgin olive oil that was utilized on monovariety oils from the single, self-sterile cultivar ‘Ogliarola salentina'. We show that DNA purified from oil can be used for microsatellite analysis and that the profile of DNA purified from a monovariety oil corresponds to the profile of DNA purified from the leaves of the same cultivar. While DNA from the pollinators present in the genome of the seed embryo, could potentially contain alleles not present in the genome fruit pulp, invalidating the molecular traceability of olive oil, we show for the first time that there is no contamination of seed embryo DNA in a monovariety oil. Thus, this molecular assay is applicable for monovariety olive oils. Springer-Verlag, 2006 DNA extraction nationallicence Cultivar identification nationallicence Microsatellites nationallicence Olea europaea nationallicence Olive oil nationallicence Muzzalupo Innocenzo Consiglio per la Ricerca e Sperimentazione in Agricoltura, Istituto Sperimentale per l'Olivicoltura (CRA-ISOl), c.da Li Rocchi, 87036, Rende (CS), Italy aut Pellegrino Massimiliano Consiglio per la Ricerca e Sperimentazione in Agricoltura, Istituto Sperimentale per l'Olivicoltura (CRA-ISOl), c.da Li Rocchi, 87036, Rende (CS), Italy aut Perri Enzo Consiglio per la Ricerca e Sperimentazione in Agricoltura, Istituto Sperimentale per l'Olivicoltura (CRA-ISOl), c.da Li Rocchi, 87036, Rende (CS), Italy aut European Food Research and Technology Springer-Verlag 224/4(2007-02-01), 469-475 1438-2377 224:4<469 2007 224 217 https://doi.org/10.1007/s00217-006-0340-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0340-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Muzzalupo Innocenzo Consiglio per la Ricerca e Sperimentazione in Agricoltura, Istituto Sperimentale per l'Olivicoltura (CRA-ISOl), c.da Li Rocchi, 87036, Rende (CS), Italy aut NATIONALLICENCE 700 1- Pellegrino Massimiliano Consiglio per la Ricerca e Sperimentazione in Agricoltura, Istituto Sperimentale per l'Olivicoltura (CRA-ISOl), c.da Li Rocchi, 87036, Rende (CS), Italy aut NATIONALLICENCE 700 1- Perri Enzo Consiglio per la Ricerca e Sperimentazione in Agricoltura, Istituto Sperimentale per l'Olivicoltura (CRA-ISOl), c.da Li Rocchi, 87036, Rende (CS), Italy aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 224/4(2007-02-01), 469-475 1438-2377 224:4<469 2007 224 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer