caa a22 4500 46315746X CHVBK 20180406164738.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s00217-006-0306-0 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0306-0 Screening of free radical scavenging capacity and antioxidant activities of Rosmarinus officinalis extracts with focus on location and harvesting times [Elektronische Daten] [O. Yesil-Celiktas, G. Girgin, H. Orhan, H. Wichers, E. Bedir, F. Vardar-Sukan] Methanolic extracts from the leaves of Rosmarinus officinalis (rosemary) harvested from different locations of Turkey at four different times of the year were analyzed by HPLC, and their radical scavenging capacities and antioxidant activities were studied by various assays. The amounts of carnosol, carnosic acid and rosmarinic acid, active constituents of rosemary, varied in different geographical regions of growth, and also showed a seasonal variation. The levels of the constituents were higher in the warm months of June 2004 and September 2004. The antioxidant activities of 12 extracts were determined by in vitro DPPH radical scavenging activity, by Trolox equivalent antioxidant capacity (TEAC), and by reversing H2O2-induced erythrocyte membrane lipid peroxidation (EMLP). The two antioxidant enzyme activities of human erythrocyte, namely superoxide dismutase (SOD) and catalase (CAT), after in vitro incubation with the extracts, were also examined in order to see whether the observed effects are related to altered enzymatic efficiency. The resulting values were correlated with active metabolite and total phenol contents of the extracts. The results indicated that the plants harvested in September possessing higher levels of active constituent had superior antioxidant capacities compared to the ones collected at other times. With respect to the location, plants harvested from the Izmir region had lower total phenol and active constituent levels resulting in poorer antioxidant activity. Springer-Verlag, 2006 Rosmarinus officinalis nationallicence Antioxidant activity nationallicence Radical scavenging nationallicence DPPH nationallicence TEAC nationallicence Lipid peroxidation nationallicence Malondialdehyde nationallicence SOD nationallicence CAT nationallicence Yesil-Celiktas O. Ege University Science and Technology Center, 35100, Bornova-Izmir, Turkey aut Girgin G. Department of Toxicology, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey aut Orhan H. Department of Toxicology, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey aut Wichers H. Agrotechnology and Food Innovations Institute, Wageningen University, 6700, AA Wageningen, The Netherlands aut Bedir E. Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova-Izmir, Turkey aut Vardar-Sukan F. Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova-Izmir, Turkey aut European Food Research and Technology Springer-Verlag 224/4(2007-02-01), 443-451 1438-2377 224:4<443 2007 224 217 https://doi.org/10.1007/s00217-006-0306-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0306-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yesil-Celiktas O. Ege University Science and Technology Center, 35100, Bornova-Izmir, Turkey aut NATIONALLICENCE 700 1- Girgin G. Department of Toxicology, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey aut NATIONALLICENCE 700 1- Orhan H. Department of Toxicology, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey aut NATIONALLICENCE 700 1- Wichers H. Agrotechnology and Food Innovations Institute, Wageningen University, 6700, AA Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Bedir E. Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova-Izmir, Turkey aut NATIONALLICENCE 700 1- Vardar-Sukan F. Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova-Izmir, Turkey aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 224/4(2007-02-01), 443-451 1438-2377 224:4<443 2007 224 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer