caa a22 4500 463157524 CHVBK 20180406164738.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s00217-006-0358-1 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0358-1 Inhibitory activity of ginger rhizome on airway and uterine smooth muscle preparations [Elektronische Daten] [Muhammad Ghayur, Anwarul Gilani] In this study, we describe the tracheal-relaxant and tocolytic activities of the methanol (ME) and aqueous (AQ) crude extracts of ginger (rhizome of Zingiber officinale) in an attempt to rationalize its traditional use in disorders of airways and uterine hyperactivity. Both of the ginger extracts dose-dependently relaxed K+ (80mM) and carbachol (CCh, 1μM)-induced contractions with more potency against K+, similar to that elicited by verapamil, a Ca2+ channel blocker. In isolated uterine preparations, the extracts suppressed the K+-induced contractions with respective EC50 values of 0.03mg/ml (0.02-0.05, 95% CI) and 0.05mg/ml (0.04-0.06). Activity-directed fractionation of AQ yielded an organic and an aqueous fraction with the activities concentrated in the former. Both the crude extracts were found safe in mice up to the oral dose of 5g/kg when tested for acute toxicity for 24h. The study shows that ginger possesses tracheal and uterine smooth muscle relaxant activity, possibility mediated via Ca2+ channel blockade, justifying its use in disorders such as asthma, cough as well as in dysmenorrhoea and uterine and menstrual spasms and congestion. Springer-Verlag, 2006 Zingiber officinale nationallicence Ginger nationallicence Tracheal-relaxant nationallicence Tocolytic nationallicence Ca2+ antagonist nationallicence ME : Ginger methanolic crude extract nationallicence AQ : Ginger aqueous crude extract nationallicence AQ.PE : Petroleum ether fraction of ginger aqueous crude extract nationallicence AQ.AQ : Aqueous fraction of ginger aqueous crude extract nationallicence CCB : Calcium channel blocker nationallicence ACh : Acetylcholine nationallicence CCh : Carbachol nationallicence Ghayur Muhammad Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, 74800, Karachi, Pakistan aut Gilani Anwarul Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, 74800, Karachi, Pakistan aut European Food Research and Technology Springer-Verlag 224/4(2007-02-01), 477-481 1438-2377 224:4<477 2007 224 217 https://doi.org/10.1007/s00217-006-0358-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0358-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ghayur Muhammad Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, 74800, Karachi, Pakistan aut NATIONALLICENCE 700 1- Gilani Anwarul Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, 74800, Karachi, Pakistan aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 224/4(2007-02-01), 477-481 1438-2377 224:4<477 2007 224 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer