caa a22 4500 463157710 CHVBK 20180406164739.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s00217-006-0348-3 doi (NATIONALLICENCE)springer-10.1007/s00217-006-0348-3 Blocking agents for ELISA quantification of compounds coming from bovine muscle crude extracts [Elektronische Daten] [Miguel Sentandreu, Laurent Aubry, Fidel Toldrá, Ahmed Ouali] Non-specific binding (NSB) of compounds other than the capture antigen or antibody to the solid support is one of the most important error sources occurring in ELISA. Undesirable NSB may be minimized by saturating the remaining binding sites of the plastic surface with different protein compounds (blocking agents) that must not interfere with the immunoassay. This work had as main objective the evaluation of different blocking agents for their ability to avoid unwanted NSB in ELISA using bovine muscle crude extracts and a polyclonal antibody raised against purified cathepsin L. Results obtained showed that skimmed milk, casein, BSA, goat and rabbit serum proved to be efficient in suppressing NSB. In the case of skimmed milk and casein, an effective blocking effect was observed even in absence of the surcoating step. However, these two compounds and BSA interfered considerably the immunochemical reaction, making their use inadequate. Of the two assayed serums, rabbit serum would be a more suitable blocking agent than goat serum when working with bovine crude extracts because it did not interfere in great manner with the antigen-antibody reaction and, at the same time, did not increase background. Springer-Verlag, 2006 Blocking agents nationallicence Muscle nationallicence Immunoassays nationallicence Meat quality nationallicence ELISA nationallicence Cathepsin L nationallicence Sentandreu Miguel Instituto de Agroquímica y Tecnología de Alimentos (CSIC), 46100 Burjassot, P.O. Box 73, Valencia, Spain aut Aubry Laurent Muscle Biochemistry Group, Qualité des Produits Animaux, INRA-Theix, 63122, Saint Genès Champanelle, France aut Toldrá Fidel Instituto de Agroquímica y Tecnología de Alimentos (CSIC), 46100 Burjassot, P.O. Box 73, Valencia, Spain aut Ouali Ahmed Muscle Biochemistry Group, Qualité des Produits Animaux, INRA-Theix, 63122, Saint Genès Champanelle, France aut European Food Research and Technology Springer-Verlag 224/5(2007-03-01), 623-628 1438-2377 224:5<623 2007 224 217 https://doi.org/10.1007/s00217-006-0348-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00217-006-0348-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sentandreu Miguel Instituto de Agroquímica y Tecnología de Alimentos (CSIC), 46100 Burjassot, P.O. Box 73, Valencia, Spain aut NATIONALLICENCE 700 1- Aubry Laurent Muscle Biochemistry Group, Qualité des Produits Animaux, INRA-Theix, 63122, Saint Genès Champanelle, France aut NATIONALLICENCE 700 1- Toldrá Fidel Instituto de Agroquímica y Tecnología de Alimentos (CSIC), 46100 Burjassot, P.O. Box 73, Valencia, Spain aut NATIONALLICENCE 700 1- Ouali Ahmed Muscle Biochemistry Group, Qualité des Produits Animaux, INRA-Theix, 63122, Saint Genès Champanelle, France aut NATIONALLICENCE 773 0- European Food Research and Technology Springer-Verlag 224/5(2007-03-01), 623-628 1438-2377 224:5<623 2007 224 217 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer