caa a22 4500 463159381 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0006-z doi (NATIONALLICENCE)springer-10.1007/s10535-007-0006-z Development of the CELLOP optimisation model for plant cell cultivation [Elektronische Daten] [M. Eeva, P. Vuorela, P. Tammela, M. Nyman, S. Ojala, K. Fagerstedt, H. Haario, H. Vuorela] A mathematical computer-aided model CELLOP was constructed in which the desirability functions in a three-dimensional experimental design can be used to find the optimal growing conditions for plant cells. CELLOP is intended for the optimisation of 3 to 6 physical, chemical, or biological variables in the cultivation conditions of plant cell cultures. The model was used to optimise the culturing conditions (calcium, inorganic nitrogen, and sucrose concentrations) for coumarin-producing, spontaneously embryogenic cell lines of angelica Angelica archangelica L. subsp. archangelica and hogs fennel Peucedanum palustre (L.) Moench. For A. archangelica the overall optimum concentrations were 0.47 mM Ca2+, 5.06 mM NO3 −, 0.40 mM NH4 +, and 96.25 mM sucrose. The dry mass was 24.7 % higher and the coumarin content 40.5 % higher than those achieved in the standard 75 % Gamborg B5 medium. For A. archangelica the highest embryogenic activity was reached in the media containing 1.25 mM Ca2+. In the case of P. palustre the overall optimum concentrations were 1.60 mM Ca2+, 2.84 mM NO3 −, 0.23 mM NH4 +, and 85.10 mM sucrose. For P. palustre the dry mass production increased by 61.8 % and the coumarin content by 58.1 % compared to the values achieved in the Gamborg B5 medium. For P. palustre the highest embryogenic activity was reached in the presence of 50.0 mM NO3 − and 4.01 mM NH4 +. Institute of Experimental Botany, ASCR, 2007 Angelica archangelica nationallicence desirability function nationallicence embryogenic cell lines nationallicence Peucedanum palustre nationallicence Eeva M. Faculty of Pharmacy, Department of Pharmaceutical Biology, University of Helsinki, P.O. Box 56, FIN-00014, Helsinki, Finland aut Vuorela P. Department of Biochemistry and Pharmacy, Biocity, Åbo Akademi University, FIN-20520, Turku, Finland aut Tammela P. Drug Discovery and Development Technology Center, Faculty of Pharmacy, University of Helsinki, FIN-00014, Finland aut Nyman M. Department of Biological and Environmental Sciences, Viikki Biocenter, University of Helsinki, FIN-00014, Finland aut Ojala S. Faculty of Pharmacy, Department of Pharmaceutical Biology, University of Helsinki, P.O. Box 56, FIN-00014, Helsinki, Finland aut Fagerstedt K. Department of Biological and Environmental Sciences, Viikki Biocenter, University of Helsinki, FIN-00014, Finland aut Haario H. Department of Mathematics, University of Helsinki, FIN-00014, Finland aut Vuorela H. Faculty of Pharmacy, Department of Pharmaceutical Biology, University of Helsinki, P.O. Box 56, FIN-00014, Helsinki, Finland aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 27-33 0006-3134 51:1<27 2007 51 10535 https://doi.org/10.1007/s10535-007-0006-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0006-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Eeva M. Faculty of Pharmacy, Department of Pharmaceutical Biology, University of Helsinki, P.O. Box 56, FIN-00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Vuorela P. Department of Biochemistry and Pharmacy, Biocity, Åbo Akademi University, FIN-20520, Turku, Finland aut NATIONALLICENCE 700 1- Tammela P. Drug Discovery and Development Technology Center, Faculty of Pharmacy, University of Helsinki, FIN-00014, Finland aut NATIONALLICENCE 700 1- Nyman M. Department of Biological and Environmental Sciences, Viikki Biocenter, University of Helsinki, FIN-00014, Finland aut NATIONALLICENCE 700 1- Ojala S. Faculty of Pharmacy, Department of Pharmaceutical Biology, University of Helsinki, P.O. Box 56, FIN-00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Fagerstedt K. Department of Biological and Environmental Sciences, Viikki Biocenter, University of Helsinki, FIN-00014, Finland aut NATIONALLICENCE 700 1- Haario H. Department of Mathematics, University of Helsinki, FIN-00014, Finland aut NATIONALLICENCE 700 1- Vuorela H. Faculty of Pharmacy, Department of Pharmaceutical Biology, University of Helsinki, P.O. Box 56, FIN-00014, Helsinki, Finland aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 27-33 0006-3134 51:1<27 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer