caa a22 4500 46315939X CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0027-7 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0027-7 Agrobacterium -mediated transformation of indica rice with chitinase gene for enhanced sheath blight resistance [Elektronische Daten] [R. Nandakumar, S. Babu, K. Kalpana, T. Raguchander, P. Balasubramanian, R. Samiyappan] Four rice indica genotypes of local importance were transformed with RC7, rice chitinase cDNA clone through Agrobacterium-mediated gene transfer method using mature seed derived calli as explants. The putative hygromycin resistant calli showed varied level of regeneration efficiency ranging from 2.0 to 7.6 %. The stable integration and expression of RC7 was confirmed through polymerase chain reaction (PCR) and Western analysis. Transformation efficiency ranged from 0.9 to 5.2 %. The expression of RC7 (35 kDa chitinase) in different tissues of transgenic plant (root, sheath and leaf) was proved through Western analysis and in terms of increased chitinase activity. The inheritance of transgene was studied through PCR and Western analysis in transgenic plants of Pusa Basmati 1. Bioassays with transgenic plants of local cultivars exhibited enhanced resistance up to 33.3 % to rice sheath blight pathogen Rhizoctonia solani under glasshouse conditions. Enhanced expression or 3-to 4-fold increased activity of chitinase in transgenic plants was correlated with sheath blight resistance. Institute of Experimental Botany, ASCR, 2007 antifungal activity nationallicence Oryza sativa nationallicence PCR nationallicence Rhizoctonia solani nationallicence bar : biolaphos resistance gene nationallicence 2,4-D : 2,4-dichlorophenoxy acetic acid nationallicence hph : hygromycin resistance gene nationallicence MS medium : Murashige and Skoog medium nationallicence NAA : naphthalene acetic acid (NAA) nationallicence PCR : polymerase chain reaction nationallicence PDI : percent disease index nationallicence PR : protein-pathogenesis-related proteins nationallicence Nandakumar R. Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut Babu S. Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut Kalpana K. Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut Raguchander T. Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut Balasubramanian P. Rice Transformation Laboratory, Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut Samiyappan R. Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 142-148 0006-3134 51:1<142 2007 51 10535 https://doi.org/10.1007/s10535-007-0027-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0027-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nandakumar R. Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut NATIONALLICENCE 700 1- Babu S. Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut NATIONALLICENCE 700 1- Kalpana K. Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut NATIONALLICENCE 700 1- Raguchander T. Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut NATIONALLICENCE 700 1- Balasubramanian P. Rice Transformation Laboratory, Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut NATIONALLICENCE 700 1- Samiyappan R. Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, 641003, Coimbatore, India aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 142-148 0006-3134 51:1<142 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer