caa a22 4500 463159446 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0035-7 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0035-7 Sotiropoulos T. Pomology Institute, N.AG.RE.F., P.O. Box 122, GR-59200, Naoussa, Greece aut Effect of NaCl and CaCl2 on growth and contents of minerals, chlorophyll, proline and sugars in the apple rootstock M 4 cultured in vitro [Elektronische Daten] [T. Sotiropoulos] The apple (Malus domestica Borkh) rootstock M 4 shoots were grown in vitro for 4 weeks on Murashige and Skoog (MS) medium containing three NaCl concentrations (35, 100 and 200 mM) in combination with two CaCl2 concentrations (5 and 10 mM). Inclusion of 10 mM CaCl2 in the medium, in the presence of 35 mM NaCl, significantly increased the number of shoots and the fresh mass compared to 5 mM CaCl2. The number of shoots, length of shoots, and the fresh mass of cultures were very low in the presence of 100 and 200 mM NaCl, independently of CaCl2 concentration of the medium. By increasing NaCl and CaCl2 concentrations in the culture medium, contents of N, Na, Cl, proline and soluble sugars in plantlets increased, whereas K, Mg, B, Zn and chlorophyll content decreased in comparison to the control. Institute of Experimental Botany, ASCR, 2007 cell proliferation nationallicence ion toxicity nationallicence micropropagation nationallicence d.m. : dry mass nationallicence f.m. : fresh mass nationallicence MS medium : Murashige and Skoog medium nationallicence Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 177-180 0006-3134 51:1<177 2007 51 10535 https://doi.org/10.1007/s10535-007-0035-7 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0035-7 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Sotiropoulos T. Pomology Institute, N.AG.RE.F., P.O. Box 122, GR-59200, Naoussa, Greece aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 177-180 0006-3134 51:1<177 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer