caa a22 4500 463159470 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0011-2 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0011-2 Decreased amount of reducing sugars in transgenic potato tubers and its influence on yield characteristics [Elektronische Daten] [O. Navrátil, L. Fischer, J. Čmejlová, M. Linhart, J. Vacek] This work focuses on the comparison of field characteristics and amounts of reducing sugars in cold-stored tubers of transgenic plants derived from two potato cultivars. The bacterial gene coding for phosphofructokinase under the tuber-specific promoter was used to support the glycolysis in stored tubers. While the tubers from untransformed control plants steadily accumulated reducing sugars during cold storage, the tubers from transformed plants regardless the genotype were characterized by subsequent decrease in the sugar content. After long period of cold storage the greatest reduction in the reducing sugar content was by more than 60 % compared to control. Before the storage, however, the content of reducing sugars was in 80 % of transgenic lines higher than in control ones. The plants evaluated in field trials for their appearance showed any changes in growth characteristics in about 25 % of the transgenic lines. Despite the introduced modification of sugar metabolism the yield of transgenic plants with normal appearance did not differ significantly from the yield of control plants. Institute of Experimental Botany, ASCR, 2007 Lactobacillus bulgaricus nationallicence low temperature sweetening nationallicence phosphofructokinase nationallicence FM : fresh mass nationallicence LbPFK : phosphofructokinase from Lactobacillus bulgaricus nationallicence LTS : low temperature sweetening nationallicence MS medium : Murashige and Skoog medium nationallicence PFK : phosphofructokinase nationallicence Navrátil O. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, CZ-16502, Praha 6, Czech Republic aut Fischer L. Faculty of Sciences, Charles University, Viničná 5, CZ-12844, Praha 2, Czech Republic aut Čmejlová J. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, CZ-16502, Praha 6, Czech Republic aut Linhart M. Sativa Keřkov, Dobrovského 3538, CZ-580 03, Havlíčkův Brod, Czech Republic aut Vacek J. Potato Research Institute, Dobrovského 2366, CZ-580 01, Havlíčkův Brod, Czech Republic aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 56-60 0006-3134 51:1<56 2007 51 10535 https://doi.org/10.1007/s10535-007-0011-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0011-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Navrátil O. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, CZ-16502, Praha 6, Czech Republic aut NATIONALLICENCE 700 1- Fischer L. Faculty of Sciences, Charles University, Viničná 5, CZ-12844, Praha 2, Czech Republic aut NATIONALLICENCE 700 1- Čmejlová J. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, CZ-16502, Praha 6, Czech Republic aut NATIONALLICENCE 700 1- Linhart M. Sativa Keřkov, Dobrovského 3538, CZ-580 03, Havlíčkův Brod, Czech Republic aut NATIONALLICENCE 700 1- Vacek J. Potato Research Institute, Dobrovského 2366, CZ-580 01, Havlíčkův Brod, Czech Republic aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 56-60 0006-3134 51:1<56 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer