caa a22 4500 463159527 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0008-x doi (NATIONALLICENCE)springer-10.1007/s10535-007-0008-x Production of asiaticoside and madecassoside in Centella asiatica in vitro and in vivo [Elektronische Daten] [Z. Aziz, M. Davey, J. Power, P. Anthony, R. Smith, K. Lowe] The localization was determined of the triterpenoids, asiaticoside and madecassoside, in different organs of glasshouse-grown plants and cultured material, including transformed roots, of two phenotypes of Centella asiatica (L.) Urban of Malaysian origin. Methanolic extracts of asiaticoside and madecassoside were prepared for gradient HPLC analysis. The two phenotypes of C. asiatica exhibited differences in terpenoid content that were tissue specific and varied between glasshouse-grown plants and tissue culture-derived material. Terpenoid content was highest in leaves, with asiaticoside (0.79 ± 0.03 and 1.15 ± 0.10 % of dry mass) and madecassoside [0.97 ± 0.06 and 1.65 ± 0.01 %(d.m.)] in the fringed (F) and smooth leaf (S) phenotypes, respectively. Roots of the F-phenotype contained the lowest content of asiaticoside [0.12 ± 0.01 %(d.m.)], whereas petioles of S-phenotype plants contained the lowest content of asiaticoside [0.16 ± 0.01 %(d.m.)] and madecassoside [0.18 ± 0.14 %(d.m.)]. Transformed roots were induced using Agrobacterium rhizogens and their growth was maximal on Murashige and Skoog basal medium supplemented with 60 g dm−3 sucrose. However, asiaticoside and madecassoside were undetectable in transformed roots and undifferentiated callus. Institute of Experimental Botany, ASCR, 2007 Agrobacterium transformation nationallicence phenotypic variation nationallicence terpenoids nationallicence ANOVA : analysis of variance nationallicence BAP : 6-benzylaminopurine nationallicence d.m. : dry mass nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence ELISA : enzyme-linked immunosorbent assay nationallicence f.m. : fresh mass nationallicence IBA : indolebutyric acid nationallicence MS : Murashige and Skoog (1962) nationallicence LB : Luria broth nationallicence NAA : α-naphthaleneacetic acid nationallicence npt II : neomycin phosphotransferase gene nationallicence NPTII : neomycin phosphotransferase protein nationallicence SEM : standard error of the mean nationallicence Aziz Z. Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, UK aut Davey M. Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, UK aut Power J. Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, UK aut Anthony P. Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, UK aut Smith R. Department of Chemistry, Loughborough University, LE11 3TU, Loughborough, UK aut Lowe K. School of Biology, University of Nottingham, NG7 2RD, University Park, Nottingham, UK aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 34-42 0006-3134 51:1<34 2007 51 10535 https://doi.org/10.1007/s10535-007-0008-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0008-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aziz Z. Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, UK aut NATIONALLICENCE 700 1- Davey M. Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, UK aut NATIONALLICENCE 700 1- Power J. Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, UK aut NATIONALLICENCE 700 1- Anthony P. Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD, Loughborough, UK aut NATIONALLICENCE 700 1- Smith R. Department of Chemistry, Loughborough University, LE11 3TU, Loughborough, UK aut NATIONALLICENCE 700 1- Lowe K. School of Biology, University of Nottingham, NG7 2RD, University Park, Nottingham, UK aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 34-42 0006-3134 51:1<34 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer