caa a22 4500 463159543 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0012-1 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0012-1 Agrobacterium -mediated high frequency transformation in dwarf recalcitrant rice cultivars [Elektronische Daten] [P. Kant, S. Kant, R. Jain, V. Chaudhury] The Agrobacterium-mediated transformation was done in rice (Oryza sativa L. var. indica) cv. HKR126 and elite cross-bred cv. Pusa Basmati1 (PB1), using strain LBA4404 containing pCAMBIA1300 cloned with gene cassettes; potato proteinase inhibitor and Bacillus thuringiensis endotoxin (plasmid JDW53) or mannitol-1-phosphate dehydrogenase (plasmid RKJ108). Co-cultivation with scutellar-calli derived from mature seeds showed stable and highly efficient transformation. In cvs. HKR126 and PB1, 35 % and 41 % of hygromycin resistant calli were obtained. The transformation efficiency in PB1 (22.0 %) was much higher than in HKR126 (12.5 %). Similarly, PB1 had higher plant regeneration efficiency than HKR126. The shoots regenerated per callus were, 3-4 in HKR126 and 5-6 in PB1. The transformation efficiency with pRKJ108 (18.6 %) was higher than pJDW53 (15.9 %). Polymerase chain reaction (PCR) analysis showed the presence of transgenes in regenerated transgenic plants of both cultivars. Institute of Experimental Botany, ASCR, 2007 Bacillus thuringiensis endotoxin nationallicence hygromycin nationallicence kinetin nationallicence napthaleneacetic acid nationallicence PCR nationallicence potato proteinase inhibitor nationallicence CH : casein hydrolysate nationallicence cry1Ab : Bacillus thuringiensis endotoxin nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence hpt : hygromycin phosphotransferase nationallicence hygR : hygromycin resistant nationallicence KIN : kinetin nationallicence MS medium : Murashige and Skoog's medium nationallicence NAA : 1-napthaleneacetic acid nationallicence pinII : potato proteinase inhibitor nationallicence Tp- mtlD : transit peptide linked mannitol-1-phosphate dehydrogenase nationallicence Kant P. J. Blaustein Institutes for Desert Research, Department of Dryland Biotechnologies, Ben-Gurion University of the Negev, 84990, Midreshet Ben-Gurion, Israel aut Kant S. J. Blaustein Institutes for Desert Research, Department of Dryland Biotechnologies, Ben-Gurion University of the Negev, 84990, Midreshet Ben-Gurion, Israel aut Jain R. Dept. of Biotechnology and Molecular Biology, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, 125004, Hisar, India aut Chaudhury V. Dept. of Biotechnology and Molecular Biology, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, 125004, Hisar, India aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 61-68 0006-3134 51:1<61 2007 51 10535 https://doi.org/10.1007/s10535-007-0012-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0012-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kant P. J. Blaustein Institutes for Desert Research, Department of Dryland Biotechnologies, Ben-Gurion University of the Negev, 84990, Midreshet Ben-Gurion, Israel aut NATIONALLICENCE 700 1- Kant S. J. Blaustein Institutes for Desert Research, Department of Dryland Biotechnologies, Ben-Gurion University of the Negev, 84990, Midreshet Ben-Gurion, Israel aut NATIONALLICENCE 700 1- Jain R. Dept. of Biotechnology and Molecular Biology, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, 125004, Hisar, India aut NATIONALLICENCE 700 1- Chaudhury V. Dept. of Biotechnology and Molecular Biology, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, 125004, Hisar, India aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 61-68 0006-3134 51:1<61 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer