caa a22 4500 463159551 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0005-0 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0005-0 Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay [Elektronische Daten] [P. Joshi, V. Dhawan] Inter simple sequence repeat (ISSR) marker assay was employed to validate the genetic fidelity of Swertia chirayita plantlets multiplied in vitro by axillary multiplication upto forty-two passages. Sixteen ISSR primers generated a total of 102 amplicons among the tissue-cultured plants. Forty-eight amplicons were amplified in the outlier (a Swertia species). The outlier (negative control) was employed to rule out the possibility that the invariant fingerprint was due to chance alone and that the ISSR technique employed was not discriminatory enough to detect the off-types. A homogenous amplification profile was observed for all the micropropagated plants. The results confirmed the clonal fidelity of the tissue culture-raised S. chirayita plantlets and corroborated the fact that axillary multiplication is the safest mode for multiplication of true to type plants. Institute of Experimental Botany, ASCR, 2007 axillary multiplication nationallicence conservation nationallicence endangered medicinal plant nationallicence molecular markers nationallicence somaclonal variations nationallicence AFLP : amplified fragment length polymorphism nationallicence dNTPs : deoxyribonucleotide triphosphates nationallicence ISSR : inter simple sequence repeat nationallicence PCR : polymarase chain reaction nationallicence RAPD : random amplification for polymorphic DNA nationallicence RFLP : restriction fragment length polymorphism nationallicence SAMPL : selectively amplified microsatellite polymorphic locus nationallicence SSR : simple sequence repeat nationallicence Taq : Thermus aquaticus nationallicence UBC : University of British Columbia nationallicence Joshi P. TERI School of Advanced Studies, Darbari Seth Block, Habitat Place, Lodhi Road, 110 003, New Delhi, India aut Dhawan V. TERI School of Advanced Studies, Darbari Seth Block, Habitat Place, Lodhi Road, 110 003, New Delhi, India aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 22-26 0006-3134 51:1<22 2007 51 10535 https://doi.org/10.1007/s10535-007-0005-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0005-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Joshi P. TERI School of Advanced Studies, Darbari Seth Block, Habitat Place, Lodhi Road, 110 003, New Delhi, India aut NATIONALLICENCE 700 1- Dhawan V. TERI School of Advanced Studies, Darbari Seth Block, Habitat Place, Lodhi Road, 110 003, New Delhi, India aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 22-26 0006-3134 51:1<22 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer