caa a22 4500 463159578 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0031-y doi (NATIONALLICENCE)springer-10.1007/s10535-007-0031-y Withanolide production by in vitro cultures of Withania somnifera and its association with differentiation [Elektronische Daten] [M. Sharada, A. Ahuja, K. Suri, S. Vij, R. Khajuria, V. Verma, A. Kumar] Withanolides-steroidal lactones, isolated from various Solanaceous plants have received considerable attention due to their potential biological activities. Five selected withanolides (withanone, withaferin A, withanolide A, withanolide B, withanolide E) were identified by HPLC-UV (DAD) — positive ion electrospray ionization mass spectroscopy in Withania somnifera (L.) Dunal cv. WSR plants and tissues cultured in vitro at different developmental phases. Cultures were established from five explants on Murashige and Skoog's medium supplemented with different plant growth regulators. Results suggest that production of withanolides is closely associated with morphological differentiation. Institute of Experimental Botany, ASCR, 2007 callus culture nationallicence plant tissue culture nationallicence Solanaceae nationallicence steroidal lactones nationallicence B5 : Gamborg's (1968) medium nationallicence BAP : 6-benzylaminopurine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence HPLC : high pressure liquid chromatography nationallicence IAA : indole-3-acetic acid nationallicence IBA : indole-3-butyric acid nationallicence KN : kinetin nationallicence MS medium : Murashige and Skoog (1962) medium nationallicence NAA : α-naphthalene acetic acid nationallicence NMR : nuclear magnetic resonance nationallicence TLC : thin layer chromatography nationallicence Sharada M. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut Ahuja A. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut Suri K. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut Vij S. Shoolini Institute of Life Sciences & Business Management, Anand Campus, 173212, HP, Solan, India aut Khajuria R. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut Verma V. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut Kumar A. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 161-164 0006-3134 51:1<161 2007 51 10535 https://doi.org/10.1007/s10535-007-0031-y text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0031-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharada M. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut NATIONALLICENCE 700 1- Ahuja A. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut NATIONALLICENCE 700 1- Suri K. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut NATIONALLICENCE 700 1- Vij S. Shoolini Institute of Life Sciences & Business Management, Anand Campus, 173212, HP, Solan, India aut NATIONALLICENCE 700 1- Khajuria R. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut NATIONALLICENCE 700 1- Verma V. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut NATIONALLICENCE 700 1- Kumar A. Regional Research Laboratory, CSIR, 180001, Jammu-Tawi, India aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 161-164 0006-3134 51:1<161 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer