caa a22 4500 463159586 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0034-8 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0034-8 Agar/galactomannan gels applied to shoot regeneration from tobacco leaves [Elektronische Daten] [N. Lucyszyn, M. Quoirin, M. Homma, M.-R. Sierakowski] This study concerns the efficacy of partial agar substitution by galactomannans as support in plant regeneration media for Nicotiana tabacum. The production of multiple shoots from leaf-derived callus and their rooting were evaluated. The galactomannans applied were obtained from Cassia fastuosa (cassia) and Cyamopsis tetragonolobus (guar gum — a commercial galactomannan) seeds. The results obtained on media solidified with mixtures of agar/galactomannan (3 g dm−3 each) gels were compared with those on media gelled with a standard concentration of agar (6 g dm−3). The in vitro performance allowed to conclude that the use of galactomannans raised the number of shoots and improved their quality. Furthermore, the length of roots and the size of leaves were significantly higher in the media solidified with agar/guar galactomannan mixtures. Institute of Experimental Botany, ASCR, 2007 alternative gelling agent nationallicence Cassia fastuosa nationallicence Cyamopsis tetragonolobus nationallicence guar gum nationallicence Nicotiana tabacum nationallicence BAP : 6-benzylaminopurine nationallicence GM : galactomannan nationallicence MS medium : Murashige and Skoog medium nationallicence ηap : apparent viscosity nationallicence Lucyszyn N. Laboratório de Biopolímeros, Departamento de Química, Universidade Federal do Paraná, CEP 81531-990, Curitiba, PR, Brazil aut Quoirin M. Laboratório de Micropropagação Vegetal, Departamento de Botânica, Universidade Federal do Paraná, CEP 81531-990, Curitiba, PR, Brazil aut Homma M. Laboratório de Biopolímeros, Departamento de Química, Universidade Federal do Paraná, CEP 81531-990, Curitiba, PR, Brazil aut Sierakowski M.-R Laboratório de Biopolímeros, Departamento de Química, Universidade Federal do Paraná, CEP 81531-990, Curitiba, PR, Brazil aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 173-176 0006-3134 51:1<173 2007 51 10535 https://doi.org/10.1007/s10535-007-0034-8 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0034-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lucyszyn N. Laboratório de Biopolímeros, Departamento de Química, Universidade Federal do Paraná, CEP 81531-990, Curitiba, PR, Brazil aut NATIONALLICENCE 700 1- Quoirin M. Laboratório de Micropropagação Vegetal, Departamento de Botânica, Universidade Federal do Paraná, CEP 81531-990, Curitiba, PR, Brazil aut NATIONALLICENCE 700 1- Homma M. Laboratório de Biopolímeros, Departamento de Química, Universidade Federal do Paraná, CEP 81531-990, Curitiba, PR, Brazil aut NATIONALLICENCE 700 1- Sierakowski M.-R Laboratório de Biopolímeros, Departamento de Química, Universidade Federal do Paraná, CEP 81531-990, Curitiba, PR, Brazil aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 173-176 0006-3134 51:1<173 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer