caa a22 4500 463159616 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0001-4 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0001-4 Origin and development of secondary somatic embryos in transformed embryogenic cultures of Medicago sativa [Elektronische Daten] [B. Uzelac, S. Ninković, A. Smigocki, S. Budimir] Non-transformed and transformed embryogenic cultures of alfalfa (Medicago sativa L. cv. Zaječarska 83), long-term maintained on growth regulator-free medium, were histologically analyzed. In all examined cultures, somatic embryos at various stages of development were observed and secondary embryos were formed in the cotyledonary, hypocotylary and radicular region of the primary embryos. Detailed histological analysis of the torpedo shape somatic embryo revealed that secondary somatic embryos arose directly from single epidermal cells of hypocotylary axis after an unequal periclinal division. Bipolar proembryos were composed of one smaller cytoplasm rich cell and one larger more vacuolated cell. Further cell division pattern was similar for both non-transformed and transformed embryos. However, multicellular origin of secondary embryos in a direct process and even from callus can not be excluded. Institute of Experimental Botany, ASCR, 2007 alfalfa nationallicence genetic transformation nationallicence histological study nationallicence GFP : green fluorescent protein nationallicence nptII : neomycin phospho-transferase II nationallicence OCII : oryzacistatin II nationallicence Uzelac B. Institute for Biological Research "Siniša Stanković”, Bulevar despota Stefana 142, 11060, Belgrade, Serbia and Montenegro aut Ninković S. Institute for Biological Research "Siniša Stanković”, Bulevar despota Stefana 142, 11060, Belgrade, Serbia and Montenegro aut Smigocki A. Molecular Plant Pathology Laboratory, USDA-ARS, 20705, Beltsville, MD, USA aut Budimir S. Institute for Biological Research "Siniša Stanković”, Bulevar despota Stefana 142, 11060, Belgrade, Serbia and Montenegro aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 1-6 0006-3134 51:1<1 2007 51 10535 https://doi.org/10.1007/s10535-007-0001-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0001-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Uzelac B. Institute for Biological Research "Siniša Stanković”, Bulevar despota Stefana 142, 11060, Belgrade, Serbia and Montenegro aut NATIONALLICENCE 700 1- Ninković S. Institute for Biological Research "Siniša Stanković”, Bulevar despota Stefana 142, 11060, Belgrade, Serbia and Montenegro aut NATIONALLICENCE 700 1- Smigocki A. Molecular Plant Pathology Laboratory, USDA-ARS, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Budimir S. Institute for Biological Research "Siniša Stanković”, Bulevar despota Stefana 142, 11060, Belgrade, Serbia and Montenegro aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 1-6 0006-3134 51:1<1 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer