caa a22 4500 463159640 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0003-2 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0003-2 Micropropagation of Juniperus phoenicea from adult plant explants and analysis of ploidy stability using flow cytometry [Elektronische Daten] [J. Loureiro, A. Capelo, G. Brito, E. Rodriguez, S. Silva, G. Pinto, C. Santos] We report here the successful micropropagation of adult Juniperus phoenicea L. with respective ploidy stability studies. Microcuttings with axillary buds were grown on five media supplemented with different growth regulator combinations. Best elongation rates were achieved on Driver and Kuniyuki (DKW) medium supplemented with kinetin alone or with naphthaleneacetic acid (NAA), while Rugini olive (OM) medium stimulated the development of new branches. Shoots growing on Murashige and Skoog (MS) medium browned and showed necrotic zones. Shoots of second to fourth subcultures usually had higher elongation rates than those of the first culture. For rooting assays, half strength DKW and OM media, different concentrations of growth regulators, auxin continuous exposure vs. dipping and the type of solid matrix were assessed. During rooting assays, two morphotypes were observed with one type having well developed internodes and the other showing hyperhydratation and no internode development. High rooting rates (40 %) were only obtained in the first morphotype shoots exposed for 5 min to 2.4 µM IBA and then transferred to OM medium without growth regulators. Plants were acclimatized in pots containing a mixture of peat and Perlite (3:2) in greenhouse with progressive reduction of relative humidity. A flow cytometric screening for major ploidy changes revealed no differences among the morphotypes and between them and the mother plant. Also the nuclear DNA content of this species was estimated for the first time using flow cytometry (2C = 24.71 pg). Institute of Experimental Botany, ASCR, 2007 in vitro culture nationallicence nuclear DNA content nationallicence plant acclimatization nationallicence rooting studies nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence BAP : benzylaminopurine nationallicence DKW : Driver and Kuniyuki medium nationallicence FCM : flow cytometry nationallicence IAA : indole-3-acetic acid nationallicence IBA : indole-3-butyric acid nationallicence KIN : kinetin nationallicence MS : Murashige and Skoog medium nationallicence NAA : naphthaleneacetic acid nationallicence OM : Rugini olive medium nationallicence PI : propidium iodide nationallicence SH : Schenk and Hildebrandt medium nationallicence TRIS : 3-(hydroxymethyl)-aminomethane nationallicence WPM : McCown Woody plant medium nationallicence Loureiro J. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Capelo A. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Brito G. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Rodriguez E. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Silva S. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Pinto G. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Santos C. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 7-14 0006-3134 51:1<7 2007 51 10535 https://doi.org/10.1007/s10535-007-0003-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0003-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Loureiro J. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Capelo A. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Brito G. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Rodriguez E. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Silva S. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Pinto G. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 700 1- Santos C. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 7-14 0006-3134 51:1<7 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer