caa a22 4500 463159667 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0010-3 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0010-3 Green fluorescent protein reveals variability in vacuoles of three plant species [Elektronische Daten] [G. Di Sansebastiano, L. Renna, M. Gigante, M. De Caroli, G. Piro, G. Dalessandro] Two vacuolar green fluorescent proteins (GFP) were stably inserted in Nicotiana tabacum and Nicotiana benthamiana genome, with unexpected difficulties, and compared with A. thaliana cv. Wassilewskaja transgenic plants expressing the same constructs. GFP fluorescence was strong in all tissues of A. thaliana but it was barely visible in Nicotiana. Confocal microscopy analysis revealed a variable distribution of the marker in those cells where GFP fluorescence was visible. The role of light dependent proteases was the variable pointing out more inter-species diversity. GFPs degradation was much higher in Nicotiana spp. than in A. thaliana. The version of GFP used appeared not to be a good vacuolar marker for Nicotiana differentiated tissues, although it can efficiently label vacuoles in protoplasts or calli. Nevertheless the sensitivity of the reporter protein can be used as an indicator of hidden characteristics of the plant vacuoles, revealing differences otherwise invisible. One of the markers in our system, GFP-Chi, evidenced a clear morphological difference in the vacuolar system of guard cells of the three species. Institute of Experimental Botany, ASCR, 2007 Arabidopsis thaliana nationallicence GFP nationallicence Nicotiana benthamiana nationallicence Nicotiana tabacum nationallicence protease nationallicence ER : endoplasmatic reticulum nationallicence GFP : green fluorescent protein nationallicence LV : lytic vacuole nationallicence PVC : pre-vacuolar compartment nationallicence SDS : sodiumdodecyl sulphate nationallicence TIP : tonoplast intrinsic protein nationallicence VSD : vacuolar sorting determinant nationallicence Di Sansebastiano G. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut Renna L. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut Gigante M. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut De Caroli M. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut Piro G. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut Dalessandro G. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 49-55 0006-3134 51:1<49 2007 51 10535 https://doi.org/10.1007/s10535-007-0010-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0010-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Di Sansebastiano G. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut NATIONALLICENCE 700 1- Renna L. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut NATIONALLICENCE 700 1- Gigante M. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut NATIONALLICENCE 700 1- De Caroli M. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut NATIONALLICENCE 700 1- Piro G. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut NATIONALLICENCE 700 1- Dalessandro G. Laboratorio di Botanica, Di.S.Te.B.A., Università di Lecce, Via prov. le Lecce-Monteroni, I-73100, Lecce, Italy aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 49-55 0006-3134 51:1<49 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer