caa a22 4500 463159683 CHVBK 20180406164743.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0018-8 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0018-8 Superoxide dismutase activity in C3 and C3/CAM intermediate species of Clusia [Elektronische Daten] [Z. Miszalski, A. Kornas, K. Gawronska, I. Ślesak, E. Niewiadomska, J. Kruk, A. Christian, E. Fischer-Schliebs, R. Krisch, U. Lüttge] The C3-CAM intermediate Clusia minor L. and the C3 obligate Clusia multiflora H.B.K. plants were exposed for 7 d to a combination of drought stress and high irradiance of about 1200 µmol m−2 s−1 for 12 h per day. In both species under these conditions a strong decrease in stomatal conductance was observed at dawn and dusk. Changes in stomatal behaviour of C. minor were accompanied by only a low nocturnal accumulation of malate and citrate. Thus, in C. minor drought stress applied in combination with high irradiance limited CAM expression, and possibly this is the main reason why C. minor prefers semi-shaded sites in the field. The mitochondrial MnSOD, in both well watered and stressed plants of two species showed strong diurnal oscillations with maximum activity at dusk. These oscillations can be explained by the engagement of mitochondria in dissipation of an excess of reducing equivalents. In plants which are able to carry out CAM metabolism tricarboxylic acid cycle is expected to be down regulated in the dark period to prevent breakdown of the entire malate and citrate. Institute of Experimental Botany, ASCR, 2007 Crassulacean acid metabolism nationallicence oxidative stress nationallicence stomata nationallicence A : antheraxanthin nationallicence BSA : bovine serum albumin nationallicence COX : cytochrome c oxidase nationallicence CuZnSOD : CuZn-superoxide dismutase nationallicence DTT : dithiothreitol nationallicence EPS : epoxidation state of the xanthophyll cycle pigments nationallicence gs : stomatal conductance nationallicence H2EDTA : ethylenediaminetetraacetic acid nationallicence HEPES : N-(2-hydroxyethyl)piperazine-N′-(ethanesulphonic acid) nationallicence MnSOD : Mn-superoxide dismutase nationallicence PAGE : polyacrylamide gel electrophoresis nationallicence PEG : polyethylene glycol nationallicence PEPC : phosphoenolpyruvate carboxylase nationallicence PFD : photon flux density nationallicence PVPP : polyvinylpolypyrrolidone nationallicence ROS : reactive oxygen species nationallicence TCA cycle : tricarboxylic acid cycle nationallicence V : violaxanthin nationallicence Z : zeaxanthin nationallicence Miszalski Z. Institute of Biology, Pedagogical University, ul. Podbrzezie 3, PL-31054, Krakow, Poland aut Kornas A. Institute of Biology, Pedagogical University, ul. Podbrzezie 3, PL-31054, Krakow, Poland aut Gawronska K. Institute of Biology, Pedagogical University, ul. Podbrzezie 3, PL-31054, Krakow, Poland aut Ślesak I. Institute of Plant Physiology, Polish Academy of Sciences, PL-30239, Krakow, Poland aut Niewiadomska E. Institute of Plant Physiology, Polish Academy of Sciences, PL-30239, Krakow, Poland aut Kruk J. Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, PL-30387, Krakow, Poland aut Christian A. Institute of Botany, Darmstadt University of Technology, D-64287, Darmstadt, Germany aut Fischer-Schliebs E. Institute of Botany, Darmstadt University of Technology, D-64287, Darmstadt, Germany aut Krisch R. Institute of Botany, Darmstadt University of Technology, D-64287, Darmstadt, Germany aut Lüttge U. Institute of Botany, Darmstadt University of Technology, D-64287, Darmstadt, Germany aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 86-92 0006-3134 51:1<86 2007 51 10535 https://doi.org/10.1007/s10535-007-0018-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0018-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miszalski Z. Institute of Biology, Pedagogical University, ul. Podbrzezie 3, PL-31054, Krakow, Poland aut NATIONALLICENCE 700 1- Kornas A. Institute of Biology, Pedagogical University, ul. Podbrzezie 3, PL-31054, Krakow, Poland aut NATIONALLICENCE 700 1- Gawronska K. Institute of Biology, Pedagogical University, ul. Podbrzezie 3, PL-31054, Krakow, Poland aut NATIONALLICENCE 700 1- Ślesak I. Institute of Plant Physiology, Polish Academy of Sciences, PL-30239, Krakow, Poland aut NATIONALLICENCE 700 1- Niewiadomska E. Institute of Plant Physiology, Polish Academy of Sciences, PL-30239, Krakow, Poland aut NATIONALLICENCE 700 1- Kruk J. Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, PL-30387, Krakow, Poland aut NATIONALLICENCE 700 1- Christian A. Institute of Botany, Darmstadt University of Technology, D-64287, Darmstadt, Germany aut NATIONALLICENCE 700 1- Fischer-Schliebs E. Institute of Botany, Darmstadt University of Technology, D-64287, Darmstadt, Germany aut NATIONALLICENCE 700 1- Krisch R. Institute of Botany, Darmstadt University of Technology, D-64287, Darmstadt, Germany aut NATIONALLICENCE 700 1- Lüttge U. Institute of Botany, Darmstadt University of Technology, D-64287, Darmstadt, Germany aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 86-92 0006-3134 51:1<86 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer