caa a22 4500 463159713 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0026-8 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0026-8 Thaumatin gene confers resistance to fungal pathogens as well as tolerance to abiotic stresses in transgenic tobacco plants [Elektronische Daten] [M. Rajam, N. Chandola, P. Saiprasad Goud, D. Singh, V. Kashyap, M. Choudhary, D. Sihachakr] We report here the development of transgenic tobacco plants with thaumatin gene of Thaumatococcus daniellii under the control of a strong constitutive promoter-CaMV 35S. Both polymerase chain reaction and genomic Southern analysis confirmed the integration of transgene. Transgenic plants exhibited enhanced resistance with delayed disease symptoms against fungal diseases caused by Pythium aphanidermatum and Rhizoctonia solani. The leaf extract from transgenic plants effectively inhibited the mycelial growth of these pathogenic fungi in vitro. The transgenic seeds exhibited higher germination percentage and seedling survival under salinity and PEG-mediated drought stress as compared to the untransformed controls. These observations suggest that thaumatin gene can confer tolerance to both fungal pathogens and abiotic stresses. Institute of Experimental Botany, ASCR, 2007 disease resistance nationallicence Nicotiana tobacum nationallicence pathogenesis-related proteins nationallicence plant transformation nationallicence BAP : 6-benzylaminopurine nationallicence MS : Murashige and Skoog nationallicence NAA : α-naphthaleneacetic acid nationallicence PCR : polymerase chain reaction nationallicence PEG : polyethylene glycol nationallicence PR-proteins : pathogenesis-related proteins nationallicence TLPs : thaumatin-like-proteins nationallicence Rajam M. Department of Genetics, Plant Polyamine and Transgenic Research Laboratory, University of Delhi-South Campus, Benito Juarez Road, 110021, New Delhi, India aut Chandola N. Department of Genetics, Plant Polyamine and Transgenic Research Laboratory, University of Delhi-South Campus, Benito Juarez Road, 110021, New Delhi, India aut Saiprasad Goud P. Department of Genetics, Plant Polyamine and Transgenic Research Laboratory, University of Delhi-South Campus, Benito Juarez Road, 110021, New Delhi, India aut Singh D. Department of Genetics, Plant Polyamine and Transgenic Research Laboratory, University of Delhi-South Campus, Benito Juarez Road, 110021, New Delhi, India aut Kashyap V. Department of Genetics, Plant Polyamine and Transgenic Research Laboratory, University of Delhi-South Campus, Benito Juarez Road, 110021, New Delhi, India aut Choudhary M. Division of Floriculture, Indian Agricultural Research Institute, 110012, NewDelhi, India aut Sihachakr D. Lab. Ecologie, Systematique et Evolution, Université Paris Sud, F-91405, Orsay Cedex, France aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 135-141 0006-3134 51:1<135 2007 51 10535 https://doi.org/10.1007/s10535-007-0026-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0026-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rajam M. Department of Genetics, Plant Polyamine and Transgenic Research Laboratory, University of Delhi-South Campus, Benito Juarez Road, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Chandola N. Department of Genetics, Plant Polyamine and Transgenic Research Laboratory, University of Delhi-South Campus, Benito Juarez Road, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Saiprasad Goud P. Department of Genetics, Plant Polyamine and Transgenic Research Laboratory, University of Delhi-South Campus, Benito Juarez Road, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Singh D. Department of Genetics, Plant Polyamine and Transgenic Research Laboratory, University of Delhi-South Campus, Benito Juarez Road, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Kashyap V. Department of Genetics, Plant Polyamine and Transgenic Research Laboratory, University of Delhi-South Campus, Benito Juarez Road, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Choudhary M. Division of Floriculture, Indian Agricultural Research Institute, 110012, NewDelhi, India aut NATIONALLICENCE 700 1- Sihachakr D. Lab. Ecologie, Systematique et Evolution, Université Paris Sud, F-91405, Orsay Cedex, France aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 135-141 0006-3134 51:1<135 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer