caa a22 4500 463159748 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0014-z doi (NATIONALLICENCE)springer-10.1007/s10535-007-0014-z Agrobacterium tumefaciens -mediated transformation of blackgram: An assessment of factors influencing the efficiency of uidA gene transfer [Elektronische Daten] [R. Saini, P. Jaiwal] Agrobacterium tumefaciens strain EHA105 carrying a binary vector pCAMBIA2301, which contains a neomycin phosphotransferase gene (nptII) and a β-glucuronidase (GUS) gene (uidA) interrupted with an intron, was used for transformation of Vigna mungo cotyledonary node explants. Various factors such as preculture and wounding of explants, manipulations in inoculation and co-cultivation conditions were found to play a significant role in influencing tissue competence, Agrobacterium virulence and compatibility of both, for achieving the maximum transformation frequencies. The stable transformation with 4.31 % efficiency was achieved using the optimized conditions. The transformed green shoots that were selected and rooted on medium containing kanamycin and tested positive for nptII gene by polymerase chain reaction were established in soil to collect seeds. GUS activity was detected in leaves, roots, pollen grains and T1 seedlings. Southern analysis of T0 plants showed the integration of nptII into the plant genome. Institute of Experimental Botany, ASCR, 2007 cotyledonary node nationallicence GUS activity nationallicence transgenic plants nationallicence Vigna mungo nationallicence BAP : 6-benzylaminopurine nationallicence CTAB : cetyl trimethyl ammonium bromide nationallicence IBA : indole-3-butyric acid nationallicence RM : rooting medium nationallicence SR : shoot regeneration medium nationallicence Saini R. Department of Biosciences, M.D. University, 124001, Rohtak, India aut Jaiwal P. Department of Biosciences, M.D. University, 124001, Rohtak, India aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 69-74 0006-3134 51:1<69 2007 51 10535 https://doi.org/10.1007/s10535-007-0014-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0014-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saini R. Department of Biosciences, M.D. University, 124001, Rohtak, India aut NATIONALLICENCE 700 1- Jaiwal P. Department of Biosciences, M.D. University, 124001, Rohtak, India aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 69-74 0006-3134 51:1<69 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer