caa a22 4500 463159772 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s10535-007-0004-1 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0004-1 Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica [Elektronische Daten] [T. Feyissa, M. Welander, L. Negash] Randomly amplified polymorphic DNA (RAPD) markers were used to assess genetic stability of 80 micropropagated Hagenia abyssinica plants, 40 of axillary origin and 40 of adventitious origin. The shoots were isolated from the same mother tree and micropropagated for over two years. Among the 83 RAPD primers screened, 16 gave reproducible band patterns. These 16 primers produced 115 bands for each plant. One plant from axillary origin showed two unique bands with primer OPC-11. All other plants showed identical band patterns. Generally, there was no significant difference in the shoot multiplication rate between shoots of axillary and adventitious origin. Indole-3-acetic acid (IAA) resulted in better ex vitro rooting compared to indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA). Non-micropropagated plants that were grown in the greenhouse for about one year were better in ex vitro rooting compared to those of juvenile material and mature tree derived micropropagated plants of the same treatment. Adventitious rooting related oxygenase gene (ARRO-1) isolated from apple (Malus domestica) was not expressed in H. abyssinica using a complementary DNA representational difference analysis fragment (cDNA RDA14) as a probe. Institute of Experimental Botany, ASCR, 2007 adventitious shoots nationallicence ARRO-1 nationallicence axillary shoots nationallicence RAPD bands nationallicence RDA14 nationallicence shoot multiplication nationallicence 2-ODD : 2-oxoacid-dependent dioxygenase nationallicence ARRO-1 : adventitious rooting related oxygenase nationallicence BAP : benzylaminopurine nationallicence IAA : indole-3-acetic acid nationallicence IBA : indole-3-butyric acid nationallicence MS : Murashige and Skoog (1962) nationallicence NAA : α-naphthaleneacetic acid nationallicence PCR : polymerase chain reaction nationallicence RAPD : randomly amplified polymorphic DNA nationallicence RDA : representational difference analysis nationallicence Feyissa T. Department of Crop Science, Swedish University of Agricultural Sciences, SE-230 53, Alnarp, Sweden aut Welander M. Department of Crop Science, Swedish University of Agricultural Sciences, SE-230 53, Alnarp, Sweden aut Negash L. Department of Biology, Faculty of Science, Addis Ababa University, Addis Ababa, Ethiopia aut Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 15-21 0006-3134 51:1<15 2007 51 10535 https://doi.org/10.1007/s10535-007-0004-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0004-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feyissa T. Department of Crop Science, Swedish University of Agricultural Sciences, SE-230 53, Alnarp, Sweden aut NATIONALLICENCE 700 1- Welander M. Department of Crop Science, Swedish University of Agricultural Sciences, SE-230 53, Alnarp, Sweden aut NATIONALLICENCE 700 1- Negash L. Department of Biology, Faculty of Science, Addis Ababa University, Addis Ababa, Ethiopia aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/1(2007-03-01), 15-21 0006-3134 51:1<15 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer