caa a22 4500 463159780 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0116-7 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0116-7 A modified low copy number binary vector pUN for Agrobacterium -mediated plant transformation [Elektronische Daten] [E. Vaculková, J. Moravčíková, I. Matušíková, M. Bauer, J. Libantová] A modified low copy number plant binary vector pUN has been constructed and successfully used to clone unstable DNA sequences. The vector pUN comprises of low copy number, broad host range RK2 replicon from pBin19 and of multiple cloning site (MCS) and T-DNA region, both from a pBINPLUS-derived pLV06 vector. The absence of the ColE1 replicon in the backbone of the binary vector significantly contributed to stability of hardly clonable DNA sequences and enabled their transfer into the tobacco plants through Agrobacterium-mediated transformation. Institute of Experimental Botany, ASCR, 2007 ColE1 replicon nationallicence Ti vector nationallicence unstable DNA sequences nationallicence GUS : β-glucuronidase nationallicence MCS : multiple cloning site nationallicence NPTII, NPTIII : neomycin phosphotransferase II and III nationallicence Vaculková E. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, P.O. Box 39A, SK-95007, Nitra, Slovak Republic aut Moravčíková J. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, P.O. Box 39A, SK-95007, Nitra, Slovak Republic aut Matušíková I. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, P.O. Box 39A, SK-95007, Nitra, Slovak Republic aut Bauer M. Research Institute of Animal Production, Hlohovska 2, SK-94992, Nitra, Slovak Republic aut Libantová J. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, P.O. Box 39A, SK-95007, Nitra, Slovak Republic aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 538-540 0006-3134 51:3<538 2007 51 10535 https://doi.org/10.1007/s10535-007-0116-7 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0116-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vaculková E. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, P.O. Box 39A, SK-95007, Nitra, Slovak Republic aut NATIONALLICENCE 700 1- Moravčíková J. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, P.O. Box 39A, SK-95007, Nitra, Slovak Republic aut NATIONALLICENCE 700 1- Matušíková I. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, P.O. Box 39A, SK-95007, Nitra, Slovak Republic aut NATIONALLICENCE 700 1- Bauer M. Research Institute of Animal Production, Hlohovska 2, SK-94992, Nitra, Slovak Republic aut NATIONALLICENCE 700 1- Libantová J. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, P.O. Box 39A, SK-95007, Nitra, Slovak Republic aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 538-540 0006-3134 51:3<538 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer