caa a22 4500 463159802 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0112-y doi (NATIONALLICENCE)springer-10.1007/s10535-007-0112-y High frequency in vitro propagation of Holarrhena antidysenterica from nodal buds of mature tree [Elektronische Daten] [K. Mallikarjuna, G. Rajendrudu] An in vitro method for propagation of Holarrhena antidysenterica Wall. has been developed using nodal explants from mature trees growing in the field. Irrespective of concentrations and combinations of growth regulators used, the axillary and terminal buds sprouted and elongated when inoculated on Murashige and Skoog (MS) medium. The highest numbers of shoots were formed when sprouted shoots were subcultured from MS basal medium onto MS medium containing 2 mg dm−3 N6-benzyladenine (BA) and 0.5 mg dm−3 α-naphthalene acetic acid (NAA). The shoot number further increased upon subculture on MS medium containing 0.5 mg dm−3 BA. By repeated sub-culturing of shoots derived from nodal axillary buds, a high frequency multiplication rate was established. The elongated shoots were excised and rooted in auxin free MS basal medium. Ex vitro rooting of in vitro formed shoots was achieved upon dipping the microshoots for 2 min in 2 mg dm−3 of indole-3-butyric acid solution. Successful field establishment and high (80-90 %) survival of plants was observed. Institute of Experimental Botany, ASCR, 2007 benzyladenine nationallicence ex vitro rooting nationallicence indole-3-butyric acid nationallicence micropropagation nationallicence naphthalene acetic acid nationallicence BA : N6-benzyladenine nationallicence IBA : indole-3-butyric acid nationallicence IAA : indole-3-acetic acid nationallicence Kn : kinetin (6-furfuryl aminopurine) nationallicence NAA : α-naphthalene acetic acid nationallicence MS : Murashige and Skoog (1962) medium nationallicence TDZ : thidiazuron nationallicence Mallikarjuna K. Department of Botany, Acharya Nagarjuna University, 522 510, Nagarjuna Nagar, India aut Rajendrudu G. Department of Botany, Acharya Nagarjuna University, 522 510, Nagarjuna Nagar, India aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 525-529 0006-3134 51:3<525 2007 51 10535 https://doi.org/10.1007/s10535-007-0112-y text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0112-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mallikarjuna K. Department of Botany, Acharya Nagarjuna University, 522 510, Nagarjuna Nagar, India aut NATIONALLICENCE 700 1- Rajendrudu G. Department of Botany, Acharya Nagarjuna University, 522 510, Nagarjuna Nagar, India aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 525-529 0006-3134 51:3<525 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer