caa a22 4500 463159829 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0091-z doi (NATIONALLICENCE)springer-10.1007/s10535-007-0091-z Structural and chemical study of callus formation from leaves of Rubia tinctorum [Elektronische Daten] [N. Orbán, I. Boldizsár, K. Bóka] Dedifferentiation was monitored in Rubia tinctorum L. leaves over a 14-d period after callus induction using transmission electron microscope (TEM), high performance liquid chromatography (HPLC), spectroscopy and thin layer chromatography (TLC). Photosynthetic pigment loss of leaves took 3-5 d coinciding with the first period of anthraquinone accumulation. Callus cells were discernible in the region of the vascular bundles and wounded edges of leaves after 10-14 d. Characteristic ultrastructural alterations were manifested in vacuolization, appearance of mitochondria, amount of smooth endoplasmatic reticulum and cytoplasm, caryolympha density of nuclei and cytoplasm content of cells. There were special events in the transfer cells: unequal divisions of dedifferentiated plastids and lytic activity in the cell wall. Our results show that mesophyll cells seem to be stopped at a particular level of dedifferentiation, while transfer cells embodied in veins of leaves pass through further alterations and lead to callus formation. Findings suggest that a sort of dedifferentiation drift manifests in the various cells of R. tinctorum leaves during callus induction and depending on their specialized status they achieve different levels of dedifferentiation. Approximately 4 weeks after callus induction, root growth has started from the young calli. Institute of Experimental Botany, ASCR, 2007 anthraquinone synthesis nationallicence madder nationallicence mesophyll cells nationallicence photosynthetic pigments nationallicence transfer cells nationallicence ultrastructure nationallicence ACI : after callus induction nationallicence HPLC : high performance liquid chromatography nationallicence TEM : transmission electron microscope nationallicence TLC : thin layer chromatography nationallicence Orbán N. Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter Sétány 1/c, H-1117, Budapest, Hungary aut Boldizsár I. Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter Sétány 1/c, H-1117, Budapest, Hungary aut Bóka K. Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter Sétány 1/c, H-1117, Budapest, Hungary aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 421-429 0006-3134 51:3<421 2007 51 10535 https://doi.org/10.1007/s10535-007-0091-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0091-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Orbán N. Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter Sétány 1/c, H-1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Boldizsár I. Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter Sétány 1/c, H-1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Bóka K. Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter Sétány 1/c, H-1117, Budapest, Hungary aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 421-429 0006-3134 51:3<421 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer