caa a22 4500 463159845 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0127-4 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0127-4 Cytokinin oxidase/dehydrogenase activity as a tool in gibberellic acid/cytokinin cross talk [Elektronische Daten] [D. Todorova, I. Vaseva, J. Malbeck, A. Trávníčková, I. Macháčková, E. Karanov] Changes in endogenous cytokinin (CK) content and cytokinin oxidase/dehydrogenase activity (CKX) in response to gibberellic acid (GA3) in two pea cultivars with different life span were assessed. The control leaves of cv. Scinado, which developed faster, had higher initial cytokinin content and lower CKX activity, while opposite trend was observed in cv. Manuela with longer life span. Increased CKX and decreased CK content were detected in leaves of cv. Scinado after treatments with 0.5, 1 and 5 µM GA3. Changes in CK content and CKX activity in GA3-treated cv. Manuela leaves were reciprocal to those in cv. Scinado. CK content and CKX activity in roots were not significantly influenced by the application of GA3. The slight repression of CKX activity in some of the root samples was accompanied by increased isopentenyladenine and isopentenyladenine riboside content. Obtained results suggest that CKX was responsible for the changes in endogenous cytokinin pool in GA3-treated plants and most probably this enzyme represents an important link in GA/cytokinin cross talk. Institute of Experimental Botany, ASCR, 2007 isopentenyladenine nationallicence pea nationallicence Pisum sativum nationallicence zeatin nationallicence ABA : abscisic acid nationallicence BSA : bovine serum albumin nationallicence cis Z : cis zeatin nationallicence cis ZR : cis zeatin riboside nationallicence cis ZRP : cis zeatin riboside monophosphate nationallicence CK : cytokinin nationallicence CKX : cytokinin oxidase/dehydrogenase nationallicence GA : gibberellin nationallicence GA3 : gibberellic acid nationallicence HPLC : high performance liquid chromatography nationallicence iP : isopentenyladenine nationallicence iPR : isopentenyladenine riboside nationallicence iPRP : isopentenyladenine riboside monophosphate nationallicence PMSF : phenylmethylsulfonyl fluoride nationallicence Todorova D. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Block 21, BG-1113, Sofia, Bulgaria aut Vaseva I. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Block 21, BG-1113, Sofia, Bulgaria aut Malbeck J. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, CZ-16502, Prague 6, Czech Republic aut Trávníčková A. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, CZ-16502, Prague 6, Czech Republic aut Macháčková I. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, CZ-16502, Prague 6, Czech Republic aut Karanov E. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Block 21, BG-1113, Sofia, Bulgaria aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 579-583 0006-3134 51:3<579 2007 51 10535 https://doi.org/10.1007/s10535-007-0127-4 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0127-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Todorova D. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Block 21, BG-1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Vaseva I. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Block 21, BG-1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Malbeck J. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, CZ-16502, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Trávníčková A. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, CZ-16502, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Macháčková I. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 135, CZ-16502, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Karanov E. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Block 21, BG-1113, Sofia, Bulgaria aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 579-583 0006-3134 51:3<579 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer