caa a22 4500 463159853 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0117-6 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0117-6 Establishment of an Agrobacterium -mediated transformation system for Fortunella crassifolia [Elektronische Daten] [L. Yang, C.-J. Xu, G.-B. Hu, K.-S. Chen] Epicotyl segments of kumquat (Fortunella crassifolia Swingle cv. Jindan) were transformed with Agrobacterium tumefaciens GV3101 harboring neomycin phosphotransferase gene (npt II) containing plant expression vectors. Firstly, the explants were cultured in darkness at 25 °C on kanamycin free shoot regeneration medium (SRM) for 3 d, and then on SRM supplemented with 25 mg dm−3 kanamycin and 300 mg dm−3 cefotaxime for 20 d. Finally, they were subcultured to fresh SRM containing 50 mg dm−3 kanamycin monthly and grown under 16-h photoperiod. Sixty five kanamycin resistant shoots were regenerated from 500 epicotyl explants after four-month selection. Shoot tips of 20 strong shoots were grafted to 50-day-old kumquat seedlings and survival rate was 55 %. Among the 11 whole plants, 3 were transgenic as confirmed by Southern blotting. This is the first report on transgenic kumquat plants, and a transformation efficiency of 3.6 % was achieved. Institute of Experimental Botany, ASCR, 2007 epicotyl nationallicence kanamycin nationallicence kumquat nationallicence shoot tip grafting nationallicence PCR nationallicence Southern blotting nationallicence BA : 6-benzyladenine nationallicence KT : kinetin nationallicence MS : Murashige and Skoog nationallicence NAA : 1-naphthaleneacetic acid nationallicence npt II : neomycin phosphotransferase II gene nationallicence SRM : shoot regeneration medium nationallicence Yang L. The State Agricultural Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Zhejiang University, Huajiachi Campus, 310029, Hangzhou, P.R. China aut Xu C.-J The State Agricultural Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Zhejiang University, Huajiachi Campus, 310029, Hangzhou, P.R. China aut Hu G.-B Department of Fruit Science, South China Agricultural University, 510642, Guangzhou, P.R. China aut Chen K.-S The State Agricultural Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Zhejiang University, Huajiachi Campus, 310029, Hangzhou, P.R. China aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 541-545 0006-3134 51:3<541 2007 51 10535 https://doi.org/10.1007/s10535-007-0117-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0117-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang L. The State Agricultural Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Zhejiang University, Huajiachi Campus, 310029, Hangzhou, P.R. China aut NATIONALLICENCE 700 1- Xu C.-J The State Agricultural Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Zhejiang University, Huajiachi Campus, 310029, Hangzhou, P.R. China aut NATIONALLICENCE 700 1- Hu G.-B Department of Fruit Science, South China Agricultural University, 510642, Guangzhou, P.R. China aut NATIONALLICENCE 700 1- Chen K.-S The State Agricultural Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Zhejiang University, Huajiachi Campus, 310029, Hangzhou, P.R. China aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 541-545 0006-3134 51:3<541 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer