caa a22 4500 463159888 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0098-5 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0098-5 Hepatitis B surface antigen expression in NT-1 cells of tobacco using different expression cassettes [Elektronische Daten] [G. Sunil Kumar, T. Ganapathi, L. Srinivas, C. Revathi, V. Bapat] Nicotiana tabacum 1 (NT-1) cells were transformed with four different expression cassettes of hepatitis B surface antigen (HBsAg). The transformed nature of the cells was confirmed by polymerase chain reaction (PCR). The expression levels were assayed by enzyme linked immunosorbent assay (ELISA). The expressivities varied among the different cassettes and the maximum expression of 16.6 ng g−1(f.m.) of cells was noted in pEFEHER transformed cells. Salicylic acid (100 µM) treatment resulted in 1.8 fold increase of expression in pEFEHBS transformed cells. The effect of different concentrations of kanamycin and geneticin was studied on the growth of transformed cells and HBsAg expression. The cell growth was optimum at lower concentrations of the antibiotics, and the maximum expression was noted at 200 mg dm−3 of kanamycin. Institute of Experimental Botany, ASCR, 2007 ELISA nationallicence geneticin nationallicence jasmonic acid nationallicence kanamycin nationallicence Nicotiana tabacum nationallicence PCR nationallicence salicylic acid nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence EFE : ethylene forming enzyme gene promoter from banana nationallicence ELISA : enzyme linked immunosorbent assay nationallicence f.m. : fresh mass nationallicence HBsAg : hepatitis B surface antigen nationallicence HBS : hepatitis B surface antigen coding 's' gene nationallicence HER : hepatitis B surface antigen coding 's' gene with ER retention signal nationallicence NT-1 : Nicotiana tabacum 1 nationallicence PCR : polymerase chain reaction nationallicence SHER : hepatitis B surface antigen coding 's' gene with an N-terminal pea vicilin secretory signal and a C-terminal ER retention signal nationallicence Sunil Kumar G. Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut Ganapathi T. Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut Srinivas L. Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut Revathi C. Shantha Biotechnics Limited, 501401, Medchal, Hyderabad, India aut Bapat V. Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 467-471 0006-3134 51:3<467 2007 51 10535 https://doi.org/10.1007/s10535-007-0098-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0098-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sunil Kumar G. Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut NATIONALLICENCE 700 1- Ganapathi T. Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut NATIONALLICENCE 700 1- Srinivas L. Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut NATIONALLICENCE 700 1- Revathi C. Shantha Biotechnics Limited, 501401, Medchal, Hyderabad, India aut NATIONALLICENCE 700 1- Bapat V. Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Trombay, Mumbai, India aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 467-471 0006-3134 51:3<467 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer