caa a22 4500 463159918 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0115-8 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0115-8 Plant regeneration from Gossypium davidsonii protoplasts via somatic embryogenesis [Elektronische Daten] [X. Yang, X. Guo, X. Zhang, Y. Nie, S. Jin] Protoplasts isolated from wild cotton Gossypium davidsonii were cultured in KM8P medium supplemented with different phytohormones. The most effective combination was 0.45 µM 2,4-dichlorophenoxyacetic acid, 2.68 µM α-naphthaleneacetic acid and 0.93 µM kinetin and the division percentage at the 8th day was 30.78 ± 3.04 %. The density of protoplasts at 2-10 × 105 cm−3 was suitable for protoplast division and calli formation, with a division percentage of 32.21 ± 3.64 % and a plating efficiency of 9.12 ± 2.61 % at the 40th day. The optimal osmotic potential was achieved using 0.5 M glucose or 0.1 M glucose plus 0.5 M mannitol. Protoplasts were cultured in three ways, a double-layer culture system, with liquid over solid medium was proved to be the best way. Embryo induction was further increased by addition of 0.14 µM gibberellic acid. Institute of Experimental Botany, ASCR, 2007 cotton nationallicence 2,4-dichlorophenoxyacetic acid nationallicence gibberellic acid nationallicence kinetin nationallicence α-naphthaleneacetic acid nationallicence protoplast culture nationallicence CPW : cell and protoplast washing solution (Frearson et al. 1973) nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence FDA : fluorescein diacetate nationallicence GA3 : gibberellic acid nationallicence IBA : indole-3-butyric acid nationallicence Kn : kinetin nationallicence MES : 2-(N-morpholino)ethane sulfonic acid nationallicence MSB : MS medium (Murashige and Skoog 1962) and B5 (Gamborg et al. 1968) vitamins nationallicence NAA : α-naphthaleneacetic acid nationallicence Yang X. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Guo X. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Zhang X. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Nie Y. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Jin S. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 533-537 0006-3134 51:3<533 2007 51 10535 https://doi.org/10.1007/s10535-007-0115-8 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0115-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang X. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Guo X. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Zhang X. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Nie Y. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 700 1- Jin S. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, P.R. China aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 533-537 0006-3134 51:3<533 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer