caa a22 4500 463159926 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0099-4 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0099-4 Cell death induced by sodium nitroprusside and hydrogen peroxide in tobacco BY-2 cell suspension [Elektronische Daten] [J. Víteček, A. Wünschová, J. Petřek, V. Adam, R. Kizek, L. Havel] The interplay between nitric oxide (NO) and reactive oxygen species can lead to an induction of cell death in plants. The aim of our work was to find out if cyanide released from sodium nitroprusside (SNP; a donor of NO) could be involved in the cell death induction, which is triggered by SNP and H2O2. Cell suspension of Nicotiana tabacum L. (line BY-2) was treated with 0.5 mM SNP, 0.5 mM potassium ferricyanide (PFC; analogue of sodium nitroprusside which can not release NO) and/or by 0.5 mM glucose with 0.5 U cm−3 glucose oxidase (GGO; a donor system of H2O2). The cell death was induced only by combination of SNP and GGO. Thus cyanide released was not involved in the induction of cell death. However, SNP showed toxic effect because of decrease in activities of intracellular oxidoreductases and esterases. The cell death caused by SNP and GGO occurred within 12 h. During cell death either length or width of the cell increased. Central vacuole was formed in 20 to 40 % of cells. Most of the dead cells showed a condensed cytoplasm. Two hallmarks of programmed cell death (PCD), chromatin condensation and blebbing of nuclear periphery, were observed. However, oligonucleosomal fragmentation of DNA, another hallmark of PCD, was not detected. Institute of Experimental Botany, ASCR, 2007 Nicotiana tabacum nationallicence nitric oxide nationallicence potassium ferricyanide nationallicence programmed cell death nationallicence BAP : N6-benzylaminopurine nationallicence CTAB : hexadecyltrimethylammonium bromide nationallicence FDA : fluorescein diacetate nationallicence GGO : glucose with glucose oxidase nationallicence MTT : methylthiazolyltetrazolium bromide nationallicence PCD : programmed cell death nationallicence PFC : potassium ferricyanide nationallicence PI : propidium iodide nationallicence ROS : reactive oxygen species nationallicence SNP : sodium nitroprusside nationallicence Víteček J. Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Wünschová A. Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Petřek J. Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Adam V. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Kizek R. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Havel L. Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 472-479 0006-3134 51:3<472 2007 51 10535 https://doi.org/10.1007/s10535-007-0099-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0099-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Víteček J. Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 700 1- Wünschová A. Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 700 1- Petřek J. Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 700 1- Adam V. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 700 1- Kizek R. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 700 1- Havel L. Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 472-479 0006-3134 51:3<472 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer