caa a22 4500 463159950 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0107-8 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0107-8 Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus [Elektronische Daten] [B. Profotová, L. Burketová, O. Valentová] Accumulation of extracellular chitinases in Brassica napus plants infected with Turnip yellow mosaic virus (TYMV) and fungal pathogen Leptosphaeria maculans was studied in both compatible and incompatible interaction. Analysis of apoplast fluid by means of non-denaturing anodic and cathodic PAGE followed by in-gel detection of chitinase activity revealed a number of chitinase isozymes. TYMV induced 8 acidic and 4 basic isozymes in a systemic way. Except for one acidic and one basic isozyme, all other chitinases were also constitutively present in low amounts in mock inoculated control. In TYMV systemically infected plants, chitinases were detected in leaves expressing symptoms as well as in symptomless ones. Both virulent and avirulent L. maculans isolates induced production of chitinase isozymes in cotyledons in a time dependent manner. Some of them were present in plants constitutively and their content increased after inoculation. Three of five acidic and two of three basic isozymes responded to L. maculans infection. Chitinases started to accumulate before symptom appearance. First two acidic isozymes were detected 24 h after inoculation. The difference between compatible and incompatibe interaction reflected two basic isozymes. Institute of Experimental Botany, ASCR, 2007 oilseed rape nationallicence plant defence nationallicence PR-proteins nationallicence Turnip yellow mosaic virus nationallicence PAGE : polyacrylamide gel electrophoresis nationallicence Rf : relative mobility of proteins nationallicence TYMV : Turnip yellow mosaic virus nationallicence Profotová B. Institute of Chemical Technology Prague, Technická 5, 16628, Prague, Czech Republic aut Burketová L. Institute of Experimental Botany CAS, Na Karlovce 1a, 16000, Prague, Czech Republic aut Valentová O. Institute of Chemical Technology Prague, Technická 5, 16628, Prague, Czech Republic aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 507-513 0006-3134 51:3<507 2007 51 10535 https://doi.org/10.1007/s10535-007-0107-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0107-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Profotová B. Institute of Chemical Technology Prague, Technická 5, 16628, Prague, Czech Republic aut NATIONALLICENCE 700 1- Burketová L. Institute of Experimental Botany CAS, Na Karlovce 1a, 16000, Prague, Czech Republic aut NATIONALLICENCE 700 1- Valentová O. Institute of Chemical Technology Prague, Technická 5, 16628, Prague, Czech Republic aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 507-513 0006-3134 51:3<507 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer