caa a22 4500 463159969 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0119-4 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0119-4 Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions [Elektronische Daten] [J. Víteček, J. Petrlová, J. Petřek, V. Adam, L. Havel, K. Kramer, R. Kizek] Esterases (EC 3.1.1.x) represent a diverse group of hydrolases catalyzing the cleavage and formation of carboxyl ester bonds. Their connection with development has made them a suitable marker of development in plants. In the present work, we focused on the fluorimetric determination of the plant esterases in plant cell cultures (tobacco BY-2 cells and early somatic embryos of Norway spruce, clone 2/32) with respect to application the method for the study of programmed cell death and the influence of cadmium(II) ions on the plant cells. The programmed cell death has been triggered by sodium nitroprusside and glucose oxidase. The determination of the esterase activity by the proposed technique in a cell extract determined very small difference in enzyme activity, which was a reliable marker of metabolic changes. In addition, the esterase activity of spruce somatic embryos decreased with the increase in medium Cd concentration. Institute of Experimental Botany, ASCR, 2007 attomole detection nationallicence confocal microscopy nationallicence early somatic embryos of Norway spruce nationallicence fluorescein diacetate nationallicence fluorescence microscopy nationallicence protoplast nationallicence tobacco BY-2 nationallicence BA : N6-benzyladenine nationallicence Cd-EDTA : cadmium-ethylenediaminetetraacetic acid chelate nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence ESEs : early somatic embryos nationallicence FDA : fluorescein diacetate nationallicence GGO : glucose oxidase nationallicence IU : international unit nationallicence NO : nitric oxide nationallicence PCD : programmed cell death nationallicence PE : plant esterases nationallicence PI : propidium iodide nationallicence SNP : sodium nitroprusside nationallicence Víteček J. Department of Plant Biology, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Petrlová J. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Petřek J. Department of Plant Biology, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Adam V. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Havel L. Department of Plant Biology, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Kramer K. Grain Marketing and Production Research Center, Agricultural Research Service, US Department of Agriculture, 66502, Manhattan, KS, USA aut Kizek R. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 551-555 0006-3134 51:3<551 2007 51 10535 https://doi.org/10.1007/s10535-007-0119-4 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0119-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Víteček J. Department of Plant Biology, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 700 1- Petrlová J. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 700 1- Petřek J. Department of Plant Biology, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 700 1- Adam V. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 700 1- Havel L. Department of Plant Biology, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 700 1- Kramer K. Grain Marketing and Production Research Center, Agricultural Research Service, US Department of Agriculture, 66502, Manhattan, KS, USA aut NATIONALLICENCE 700 1- Kizek R. Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemědělská 1, CZ-61300, Brno, Czech Republic aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 551-555 0006-3134 51:3<551 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer