caa a22 4500 463160002 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0092-y doi (NATIONALLICENCE)springer-10.1007/s10535-007-0092-y Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut [Elektronische Daten] [A. Muthusamy, K. Vasanth, D. Sivasankari, B. Chandrasekar, N. Jayabalan] The embryogenic calli (EC) were obtained from hypocotyl explants of groundnut (Arachis hypogaea L.) cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) in combination with 0.5 mg dm−3 6-benzylaminopurine (BAP). The EC were exposed to γ-radiation (10-50 Gy) or treated with 1-5 mM of ethyl methane sulphonate (EMS) or sodium azide (SA). The mutated EC were subcultured on embryo induction medium containing 20 mg dm−3 2,4-D. Somatic embryos (SE) developed from these calli were transferred to MS medium supplemented with BAP (2.0 mg dm−3) and 0.5 mg dm−3 2,4-D for maturation. The well-developed embryos were cultured on germination medium consisting of MS salts with 2.0 mg dm−3 BAP and 0.25 mg dm−3 naphthaleneacetic acid (NAA). Well-developed plantlets were transferred for hardening and hardened plants produced normal flowers and set viable seeds. The fresh mass of the EC, mean number of SE per explant and regeneration percentage were higher at lower concentrations of mutagens (up to 30 Gy/3 mM). Some abnormalities in regenerated plants were observed, especially variations in leaf shape. Institute of Experimental Botany, ASCR, 2007 Arachis hypogaea nationallicence chemical mutagens nationallicence embryogenic callus nationallicence γ-radiation nationallicence growth regulators nationallicence BAP : 6-benzylaminopurine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence EC : embryogenic callus nationallicence EMS : ethyl methane sulphonate nationallicence GR : γ-radiation nationallicence Gy : Gray nationallicence MS : Murashige and Skoog nationallicence NAA : naphthaleneacetic acid nationallicence SA : sodium azide nationallicence Muthusamy A. School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut Vasanth K. School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut Sivasankari D. School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut Chandrasekar B. School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut Jayabalan N. School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 430-435 0006-3134 51:3<430 2007 51 10535 https://doi.org/10.1007/s10535-007-0092-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0092-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Muthusamy A. School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut NATIONALLICENCE 700 1- Vasanth K. School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut NATIONALLICENCE 700 1- Sivasankari D. School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut NATIONALLICENCE 700 1- Chandrasekar B. School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut NATIONALLICENCE 700 1- Jayabalan N. School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 430-435 0006-3134 51:3<430 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer