caa a22 4500 463160029 CHVBK 20180406164744.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0110-0 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0110-0 Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus [Elektronische Daten] [P. Ruanjan, S. Kertbundit, M. Juříček] Transgenic papayas carrying the papaya ringspot virus coat protein gene were inoculated with Papaya ringspot virus. Infection was monitored by evaluating symptoms and by reverse transcription polymerase chain reaction (RT-PCR). Among eight tested transgenic lines, clone G2 was found highly resistant to virus infection during 3 years of testing. Further analysis of this clone revealed complex multicopy transgene insertion with aberrant copies. The suspected post transcriptional gene silencing was confirmed by siRNA detection. While the R0 generation of G2 transgenic papaya was found to be fully resistant to the infection, Papaya ringspot virus was able to break this resistance in subsequent generations by suppressing post-transcriptional gene silencing (PTGS). Institute of Experimental Botany, ASCR, 2007 Carica papaya nationallicence reverse transcription PCR nationallicence CP : coat protein nationallicence HC-Pro : helper component protein nationallicence PRSV : Papaya ringspot virus nationallicence PTGS : post-transcriptional gene silencing nationallicence Ruanjan P. Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, 73170, Nakhon Pathom, Thailand aut Kertbundit S. Department of Plant Science, Faculty of Science, Mahidol University, Rama VI Road, 10400, Payathai, Bangkok, Thailand aut Juříček M. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Pernikářce 15, CZ-16000, Prague 6, Czech Republic aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 517-520 0006-3134 51:3<517 2007 51 10535 https://doi.org/10.1007/s10535-007-0110-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0110-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ruanjan P. Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, 73170, Nakhon Pathom, Thailand aut NATIONALLICENCE 700 1- Kertbundit S. Department of Plant Science, Faculty of Science, Mahidol University, Rama VI Road, 10400, Payathai, Bangkok, Thailand aut NATIONALLICENCE 700 1- Juříček M. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Pernikářce 15, CZ-16000, Prague 6, Czech Republic aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 517-520 0006-3134 51:3<517 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer