caa a22 4500 463160142 CHVBK 20180406164745.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0090-0 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0090-0 Somatic embryogenesis and plant regeneration of Abelmoschus esculentus through suspension culture [Elektronische Daten] [M. Ganesan, R. Chandrasekar, B. Ranjitha Kumari, N. Jayabalan] A simple and reliable protocol for regeneration of okra through somatic embryogenesis from suspension cultures has been developed. Embryogenic callus was obtained from hypocotyl explants cultured on media with Murashige and Skoog (MS) salts, Gamborg (B5) vitamins, 2.0 mg dm−3 2,4-dichlorophenoxyacetic acid (2,4-D), 1.0 mg dm−3 naphthaleneacetic acid (NAA), 25 mg dm−3 polyvinylpyrrolidone and 30 g dm−3 sucrose. More number and high frequency of healthy embryoids appeared individually in suspension culture containing MS salts, B5 vitamins, 2.0 mg dm−3 2,4-D and 1.0 mg dm−3 kinetin. Formation of cell clusters from the single cells was clearly noticed during ontogeny. Matured embryos at the cotyledonary stage were transferred to agar solidified medium for germination. The best conversion of embrya into plantlets (67.3 %) was recorded on media with half strength MS salts, B5 vitamins, 0.2 mg dm−3 benzylaminopurine (BAP) and 0.2 mg dm−3 gibberellic acid (GA3). The plantlets were transferred to soil and hardened in the plastic pots. After proper acclimatization, the plantlets regenerated through somatic embryogenesis were compared to seed grown plants to observe any variation. Institute of Experimental Botany, ASCR, 2007 auxins nationallicence cell aggregates nationallicence embryoids nationallicence liquid medium nationallicence okra nationallicence B5 : Gamborg et al. (1968) medium nationallicence BAP : benzylaminopurine nationallicence GA3 : gibberellic acid nationallicence KIN : kinetin nationallicence MS : Murashige and Skoog (1962) medium nationallicence NAA : naphthaleneacetic acid nationallicence PVP : polyvinylpyrrolidone nationallicence SH : Schenk and Hildebrandt (1972) medium nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence Ganesan M. Plant Biotechnology Unit, Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut Chandrasekar R. Plant Biotechnology Unit, Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut Ranjitha Kumari B. Plant Biotechnology Unit, Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut Jayabalan N. Plant Biotechnology Unit, Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 414-420 0006-3134 51:3<414 2007 51 10535 https://doi.org/10.1007/s10535-007-0090-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0090-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ganesan M. Plant Biotechnology Unit, Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut NATIONALLICENCE 700 1- Chandrasekar R. Plant Biotechnology Unit, Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut NATIONALLICENCE 700 1- Ranjitha Kumari B. Plant Biotechnology Unit, Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut NATIONALLICENCE 700 1- Jayabalan N. Plant Biotechnology Unit, Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 414-420 0006-3134 51:3<414 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer