caa a22 4500 463160177 CHVBK 20180406164745.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0095-8 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0095-8 Genetic differentiation of Vigna species by RAPD, URP and SSR markers [Elektronische Daten] [H. Dikshit, T. Jhang, N. Singh, K. Koundal, K. Bansal, N. Chandra, J. Tickoo, T. Sharma] Seventy genotypes belonging to 7 wild and cultivated Vigna species were genetically differentiated using randomly amplified polymorphic DNA (RAPD), universal rice primer (URP) and simple sequence repeat (SSR) markers. We identified RAPD marker, OPG13 which produced a species-specific fingerprint profile. This primer characterized all the Vigna species uniquely suggesting an insight for their co-evolution, domestication and interspecific relationship. The cluster analysis of combined data set of all the markers resulted in five major groups. Most of the genotypes belonging to cultivated species formed a specific group whereas all the wild species formed a separate cluster using unweighted paired group method with arithmetic averages and principle component analysis. The Mantel matrix correspondence test resulted in a high matrix correlation with best fit (r = 0.95) from combined marker data. Comparison of three-marker systems showed that SSR marker was more efficient in detecting genetic variability among all the Vigna species. The narrow genetic base of the V. radiata cultivars obtained in the present study emphasized that large germplasm collection should be used in Vigna improvement programme. Institute of Experimental Botany, ASCR, 2007 fingerprinting nationallicence green gram nationallicence molecular analysis nationallicence AFLP : amplified fragment length polymorphism nationallicence CTAB : cetyltrimethyl ammonium bromide nationallicence EMR : effective multiplex ratio nationallicence Hav : arithmetic mean heterozygosity nationallicence Hn : expected heterozygosity nationallicence ISSR : inter simple sequence repeats nationallicence MI : marker index nationallicence PCA : principal components analysis nationallicence PCR : polymerase chain reaction nationallicence RAPD : randomly amplified polymorphic DNA nationallicence Rp : resolving power nationallicence SAHN : sequential agglomerative hierarical nested nationallicence SAMPL : selective amplification of microsatellite polymorphic loci nationallicence SSR : simple sequence repeat nationallicence STMS : sequence tagged microsatellite nationallicence UPGMA : unweighted paired group method with arithmetic averages nationallicence URP : universal rice primer nationallicence Dikshit H. Indian Agricultural Research Institute, Division of Genetics, 110012, New Delhi, India aut Jhang T. Indian Agricultural Research Institute, National Research Center on Plant Biotechnology, 110012, New Delhi, India aut Singh N. Indian Agricultural Research Institute, National Research Center on Plant Biotechnology, 110012, New Delhi, India aut Koundal K. Indian Agricultural Research Institute, National Research Center on Plant Biotechnology, 110012, New Delhi, India aut Bansal K. Indian Agricultural Research Institute, National Research Center on Plant Biotechnology, 110012, New Delhi, India aut Chandra N. Indian Agricultural Research Institute, Division of Genetics, 110012, New Delhi, India aut Tickoo J. Indian Agricultural Research Institute, Division of Genetics, 110012, New Delhi, India aut Sharma T. Indian Agricultural Research Institute, National Research Center on Plant Biotechnology, 110012, New Delhi, India aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 451-457 0006-3134 51:3<451 2007 51 10535 https://doi.org/10.1007/s10535-007-0095-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0095-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dikshit H. Indian Agricultural Research Institute, Division of Genetics, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Jhang T. Indian Agricultural Research Institute, National Research Center on Plant Biotechnology, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Singh N. Indian Agricultural Research Institute, National Research Center on Plant Biotechnology, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Koundal K. Indian Agricultural Research Institute, National Research Center on Plant Biotechnology, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Bansal K. Indian Agricultural Research Institute, National Research Center on Plant Biotechnology, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Chandra N. Indian Agricultural Research Institute, Division of Genetics, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Tickoo J. Indian Agricultural Research Institute, Division of Genetics, 110012, New Delhi, India aut NATIONALLICENCE 700 1- Sharma T. Indian Agricultural Research Institute, National Research Center on Plant Biotechnology, 110012, New Delhi, India aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 451-457 0006-3134 51:3<451 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer