caa a22 4500 463160223 CHVBK 20180406164745.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s10535-007-0130-9 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0130-9 Photosynthetic gene expression in black willow under various soil moisture regimes [Elektronische Daten] [S. Li, S. Goodwin, S. Pezeshki] This study was the first attempt to extract RNA from black willow (Salix nigra Marshall) that contains numerous secondary products and to examine the photosynthetic gene expression of black willow under a wide range of soil moisture regimes. Black willow cuttings were grown under control, continuous flooding, periodic flooding and periodic drought for 42 d. A modified lithium chloride precipitation method was used for RNA extraction. Results of real-time polymerase chain reaction showed reduced gene expression of oxygen evolving complex, large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenasse and ferredoxin on day 7 as well as the latter two on day 14 in response to flooding. Therefore, decreased expression of these three genes may have contributed to the observed reduced photosynthetic capacity in response to flooding. Institute of Experimental Botany, ASCR, 2007 ferredoxin nationallicence large subunit of Rubisco nationallicence oxygen evolving complex nationallicence Salix nigra nationallicence C : control nationallicence CF : continuous flooding nationallicence Fd : ferredoxin nationallicence OEC : oxygen evolving complex nationallicence PCL : phenol:choroform:isoamyl alcohol nationallicence PD : periodic drought nationallicence PF : periodic flooding nationallicence PPFD : photosynthetic photon flux density nationallicence rbcL : large subunit of Rubisco nationallicence Rubisco : ribulose-1,5-bisphosphate carboxylase/oxygenasse nationallicence Li S. Department of Biology, The University of Memphis, 38152, Memphis, TN, USA aut Goodwin S. W. Harry Feinstone Center for Genomic Research, The University of Memphis, 38152, Memphis, TN, USA aut Pezeshki S. Department of Biology, The University of Memphis, 38152, Memphis, TN, USA aut Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 593-596 0006-3134 51:3<593 2007 51 10535 https://doi.org/10.1007/s10535-007-0130-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0130-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li S. Department of Biology, The University of Memphis, 38152, Memphis, TN, USA aut NATIONALLICENCE 700 1- Goodwin S. W. Harry Feinstone Center for Genomic Research, The University of Memphis, 38152, Memphis, TN, USA aut NATIONALLICENCE 700 1- Pezeshki S. Department of Biology, The University of Memphis, 38152, Memphis, TN, USA aut NATIONALLICENCE 773 0- Biologia Plantarum Kluwer Academic Publishers-Consultants Bureau 51/3(2007-09-01), 593-596 0006-3134 51:3<593 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer