caa a22 4500 463160231 CHVBK 20180406164745.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0153-2 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0153-2 Proliferation and differentiation from endosperms of Carthamus tinctorius [Elektronische Daten] [N. Walia, A. Kaur, S. Babbar] The endosperms of Carthamus tinctorius cv. HUS-305, excised at globular to heart-shaped stages of zygotic embryo development, were cultured on Murashige and Skoog's medium (MS) supplemented with different concentrations of 6-benzylaminopurine (BAP), kinetin, thidiazuron (TDZ), 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthalene-acetic acid (NAA). The highest incidence of callusing was on 2,4-D supplemented media. However, embryos differentiated only from the calli developed on media supplemented with BAP, kinetin or TDZ with the last eliciting maximum embryogenic response. The addition of a reduced nitrogen source, casein hydrolysate to MS medium supplemented with BAP and/or NAA, did not stimulate the response. However, adenine sulphate (100 mg dm−3) promoted the induction of somatic embryos. Upon transfer to MS basal medium or the same supplemented with 0.61 µM gibberellic acid (GA3), plumular poles of few embryos elongated resulting in the development of shoots. Institute of Experimental Botany, ASCR, 2007 Asteraceae nationallicence endosperm culture nationallicence growth regulators nationallicence safflower nationallicence triploids nationallicence ABA : abscisic acid nationallicence BAP : 6-benzylaminopurine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence GA3 : gibberellic acid nationallicence NAA : α-naphthaleneacetic acid nationallicence TDZ : thidiazuron nationallicence Walia N. Department of Botany, University of Delhi, 110007, Delhi, India aut Kaur A. Department of Botany, University of Delhi, 110007, Delhi, India aut Babbar S. Department of Botany, University of Delhi, 110007, Delhi, India aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 749-753 0006-3134 51:4<749 2007 51 10535 https://doi.org/10.1007/s10535-007-0153-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0153-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Walia N. Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Kaur A. Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Babbar S. Department of Botany, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 749-753 0006-3134 51:4<749 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer