caa a22 4500 46316024X CHVBK 20180406164745.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0159-9 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0159-9 Dell'Aquila A. Institute of Plant Genetics, CNR, Via Amendola 165/a, I-70126, Bari, Italy aut Pepper seed germination assessed by combined X-radiography and computer-aided imaging analysis [Elektronische Daten] [A. Dell'Aquila] A lot of pepper seeds having 87 % germination were subjected to X-ray inspection using a non lethal dose of radiation. Seeds with less than 2.7 % (on the basis of total seed area) of free space area, i.e. the spaces between embryo and endosperm, were classified as highly viable seeds (97-100 % germination) with the lowest level of abnormal seedlings. Seeds X-ray classified as good were subjected to a computerised image analysis to study seed imbibition and radicle elongation. The patterns of seed area increase, chosen as the most accurate indicator of seed swelling, resembled the triphasic curve of water uptake. The first phase was completed at 9 h followed by a second phase that varied widely in time until completion of germination between 52 and 96 h. The proportion of seeds with radicle protrusion between 52-56 h and 64-72 h assessed with the image analysis was significantly higher than that recorded using a conventional germination test. In addition, the rate of increase of seed area during the third phase of imbibition, mostly due to protrusion of the radicle tip and its growth, was highly correlated with the corresponding radicle elongation rate. Institute of Experimental Botany, ASCR, 2007 Capsicum annuum L nationallicence internal seed morphology nationallicence seed swelling nationallicence TIFF : tagged image file format nationallicence G : germination nationallicence MGT : mean germination time nationallicence Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 777-781 0006-3134 51:4<777 2007 51 10535 https://doi.org/10.1007/s10535-007-0159-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0159-9 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Dell'Aquila A. Institute of Plant Genetics, CNR, Via Amendola 165/a, I-70126, Bari, Italy aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 777-781 0006-3134 51:4<777 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer