caa a22 4500 463160312 CHVBK 20180406164745.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0147-0 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0147-0 Effect of cadmium on growth, proton extrusion and membrane potential in maize coleoptile segments [Elektronische Daten] [W. Karcz, R. Kurtyka] Cd accumulation, its effects on elongation growth of maize coleoptile segments, pH changes of their incubation medium and the membrane potential of parenchymal cells were studied. The Cd content increased significantly with exposure to increasing cadmium concentrations. Coleoptile segments accumulated the metal more efficiently in the range 10-100 µM Cd, than in the range 100-1000 µM Cd. Cd at concentrations higher than 1.0 µM produced a significant inhibition of both growth and proton extrusion. 100 µM Cd caused depolarization of the plasma membrane (PM) potential in parenchymal cells. The simultaneous treatment of maize coleoptile segments by indole-3-acetic acid (IAA) and Cd, counteracted the toxic effect of Cd on growth. Moreover, our data also showed that 100 µM Cd suppressed the characteristic IAA-induced hyperpolarization of the membrane potential, causing membrane depolarization. These results indicate that the toxic effect of Cd on growth of maize coleoptile segments might be, at least in part, caused via reduced PM H+-ATPase activity. Institute of Experimental Botany, ASCR, 2007 auxin nationallicence cadmium nationallicence elongation growth nationallicence indole-3-acetic acid nationallicence membrane depolarization nationallicence Zea mays nationallicence d.m. : dry mass nationallicence Em : membrane potential nationallicence IAA : indole-3-acetic acid nationallicence PM : plasma membrane nationallicence SGR : spontaneous growth response nationallicence Karcz W. Department of Plant Physiology, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, PL-40032, Katowice, Poland aut Kurtyka R. Department of Plant Physiology, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, PL-40032, Katowice, Poland aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 713-719 0006-3134 51:4<713 2007 51 10535 https://doi.org/10.1007/s10535-007-0147-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0147-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Karcz W. Department of Plant Physiology, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, PL-40032, Katowice, Poland aut NATIONALLICENCE 700 1- Kurtyka R. Department of Plant Physiology, Faculty of Biology and Environmental Protection, University of Silesia, Jagiellońska 28, PL-40032, Katowice, Poland aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 713-719 0006-3134 51:4<713 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer