caa a22 4500 463160320 CHVBK 20180406164745.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0163-0 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0163-0 In vitro minimum growth for conservation of Drosophyllum lusitanicum [Elektronische Daten] [S. Gonçalves, A. Romano] The present paper reports a protocol for minimum growth conservation of Drosophyllum lusitanicum (L.) Link. in vitro. Double-node cuttings were maintained for 4, 8 and 12 months at 5 or 25 °C in the dark. The effects of sucrose either alone at 5, 20, 30, 40 and 60 g dm−3 or at 20, 40 and 60 g dm−3 in combination with 20 g dm−3 mannitol, on survival and post-storage shoot multiplication efficiency were investigated. The cultures could effectively be conserved under minimum growth at 5 °C for 8 months on Murashige and Skoog's medium supplemented with 60 g dm−3 sucrose, 20 g dm−3 mannitol and 0.91 µM zeatin. Following extended conservation, the cultures could be successfully regenerated into new shoots, and they were morphologically similar to those of non-stored controls. Institute of Experimental Botany, ASCR, 2007 endangered species nationallicence germplasm conservation nationallicence osmotic stress nationallicence MS : Murashige and Skoog (1962) medium nationallicence Gonçalves S. Institute for Biotechnology and Bioengineering, Centre for Molecular and Structural Biomedicine, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut Romano A. Institute for Biotechnology and Bioengineering, Centre for Molecular and Structural Biomedicine, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 795-798 0006-3134 51:4<795 2007 51 10535 https://doi.org/10.1007/s10535-007-0163-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0163-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gonçalves S. Institute for Biotechnology and Bioengineering, Centre for Molecular and Structural Biomedicine, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 700 1- Romano A. Institute for Biotechnology and Bioengineering, Centre for Molecular and Structural Biomedicine, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 795-798 0006-3134 51:4<795 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer