caa a22 4500 463160339 CHVBK 20180406164745.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0143-4 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0143-4 Kaźmierczak A. Department of Cytophysiology, University of Łódź, Pilarskiego 14, PL-90231, Łódź, Poland aut Ethylene is a modulator of gibberellic acid-induced antheridiogenesis in Anemia phyllitidis gametophytes [Elektronische Daten] [A. Kaźmierczak] In fern (Anemia phyllitidis) gametophytes cellulose in the walls of the antheridial zone cells which was organized in clusters and spots was transformed via dispersed form to fibrillar arrangement (layered in oblique and perpendicular array in relation to the transverse direction of cell expansion) during antheridiogenesis induced by gibberellic acid (GA3) and/or enhanced by 1-aminocyclopropane-1-carboxylic acid (ACC). In the ACC-treated gametophytes, where antheridia were not induced, the cellulose was arranged in the same manner. Aminooxyacetic acid (AOA), which inhibits antheridiogenesis and development of fern gametophytes, produced in the cell walls both random and longitudinal type of organization of cellulose microfibrils, however, in the GA3/AOA-treated plants the oblique type was also observed. The total numbers of cells with perpendicular and/or oblique type of cellulose microfibrils in the GA3-, GA3/ACC-and GA3/AOA-treated gametophytes corresponded to the average number of antheridia formed. Moreover, it was found that the extracts from the gametophytes treated with GA3 or with the mixture of GA3 and ACC contained significantly less soluble sugars but more α-amylase-and endoglucanase-released sugars than the extracts from the gametophytes of the other series. Thin layer chromatography of the samples from the cell wall extracts hydrolyzed by endoglucanase contained xylose and cellobiose which suggested that these sugars built the xyloglucans, hemicellulose polymers responsible for tethering of walls of fern gametophyte cells like in higher plants. Institute of Experimental Botany, ASCR, 2007 aminooxyacetic acid nationallicence cellulose microfibrils nationallicence fern nationallicence sugars nationallicence xyloglucans nationallicence ACC : 1-aminocyclopropane-1-carboxylic acid nationallicence AOA : aminooxyacetic acid nationallicence DMSO : dimethyl sulfoxide nationallicence EDTA : (ethylenediamine)tetra acetic acid nationallicence GA3 : gibberellic acid nationallicence TBS : Tris/NaCl buffer nationallicence TLC : thin layer chromatography nationallicence TMD : Tris/MgCl2/DMSO buffer nationallicence Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 683-689 0006-3134 51:4<683 2007 51 10535 https://doi.org/10.1007/s10535-007-0143-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0143-4 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Kaźmierczak A. Department of Cytophysiology, University of Łódź, Pilarskiego 14, PL-90231, Łódź, Poland aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 683-689 0006-3134 51:4<683 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer