caa a22 4500 463160363 CHVBK 20180406164745.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0145-2 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0145-2 UV-B response of green and etiolated barley seedlings [Elektronische Daten] [I. Fedina, M. Velitchkova, K. Georgieva, K. Demirevska, L. Simova] 7-d-old etiolated and green barley seedlings (Hordeum vulgare L. cv. Alfa) were irradiated with UV-B for 30 min and then kept for 24 h in light or darkness. Chlorophyll (Chl) synthesis was inhibited by about 30 % as a result of UV-B irradiation, but there were no significant changes in photochemical activity measured by variable to maximum fluorescence ratio (Fv/Fm), quantum yield (ΦPS2) and oxygen evolution rate. Electron transport of etiolated seedlings was similar to that of green ones, nevertheless, the Chl content was more then 2-fold lower. Ribulose-1,5-bisphosphate carboxylase/oxygenase large and small subunits were diminished as a result of UV-B irradiation in etiolated and green plants, especially in those kept in the darkness. Catalase activity decreased and total superoxide dismutase activity increased in green and etiolated plants following UV-B treatment. When benzidine was used as a substrate, an isoform located between guaiacol peroxidases 2 and 3 (guaiacol peroxidase X) appeared, which was specific for UV-B treatment. As a result of irradiation, the contents of UV-B absorbing and UV-B induced compounds increased in green seedlings but not in etiolated seedlings. Institute of Experimental Botany, ASCR, 2007 chlorophyll fluorescence nationallicence flavonoids nationallicence Hordeum vulgare nationallicence oxygen evolution nationallicence ribulose-1,5-bisphosphate carboxylase/oxygenase nationallicence AOS : active oxygen species nationallicence Car : carotenoids nationallicence CAT : catalase nationallicence Chl : chlorophyll nationallicence GXP : guaiacol peroxidase nationallicence F0 : chlorophyll fluorescence of dark-adapted state nationallicence Fm : maximal fluorescence of PS 2 in dark-adapted state nationallicence Fv : variable chlorophyll fluorescence nationallicence qN : non-photochemical fluorescence quenching nationallicence qP : photochemical quenching nationallicence PMSF : phenylmethylsulfonyl fluoride nationallicence PPFD : photosynthetic photon flux density nationallicence PS : photosystem nationallicence RLS and RSS : ribulose-1,5-bisphosphate carboxylase/oxygenase large and small subunits nationallicence SOD : superoxide dismutase nationallicence TCA : trichloracetic acid nationallicence ΦPS2 : the quantum yield of PS 2 photochemistry in the light-adapted state nationallicence UV-B : ultraviolet B radiation nationallicence Fedina I. Institute of Plant Physiology, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street 21, BG-1113, Sofia, Bulgaria aut Velitchkova M. Institute of Biophysics, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street 21, BG-1113, Sofia, Bulgaria aut Georgieva K. Institute of Plant Physiology, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street 21, BG-1113, Sofia, Bulgaria aut Demirevska K. Institute of Plant Physiology, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street 21, BG-1113, Sofia, Bulgaria aut Simova L. Institute of Plant Physiology, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street 21, BG-1113, Sofia, Bulgaria aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 699-706 0006-3134 51:4<699 2007 51 10535 https://doi.org/10.1007/s10535-007-0145-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0145-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fedina I. Institute of Plant Physiology, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street 21, BG-1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Velitchkova M. Institute of Biophysics, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street 21, BG-1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Georgieva K. Institute of Plant Physiology, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street 21, BG-1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Demirevska K. Institute of Plant Physiology, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street 21, BG-1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Simova L. Institute of Plant Physiology, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street 21, BG-1113, Sofia, Bulgaria aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 699-706 0006-3134 51:4<699 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer