caa a22 4500 463160371 CHVBK 20180406164745.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0144-3 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0144-3 Ultrastructural alterations in mesophyll and bundle sheath chloroplasts of two maize cultivars in response to chilling at high irradiance [Elektronische Daten] [A. Saropulos, D. Drennan] Maize (Zea mays L.) seedlings of two cultivars (cv. Bastion adapted to W. Europe, and cv. Batan 8686 adapted to the highlands of Mexico), raised in a glasshouse (19-25 °C), were transferred to 4.5 or 9 °C at photon flux density (PPFD) of 950 µmol m−2 s−1 with 10-h photoperiod for 58 h and then allowed to recover at 22 °C for 16 h (14 h dark and 2 h at PPFD of 180 µmol m−2 s−1). The ultrastructural responses after 4 h or 26 h at 4.5 °C were the disappearance of starch grains in the bundle sheath chloroplasts and the contraction of intrathylakoid spaces in stromal thylakoids of the mesophyll chloroplasts. At this time, bundle sheath chloroplasts of cv. Batan 8686 formed peripheral reticulum. Prolonged stress at 4.5 °C (50 h) caused plastid swelling and the dilation of intrathylakoid spaces, mainly in mesophyll chloroplasts. Bundle sheath chloroplasts of cv. Batan 8686 seedlings appeared well preserved in shape and structure. Batan 8686 had also higher net photosynthetic rates during chilling and recovery than Bastion. Extended leaf photobleaching developed during the recovery period after chilling at 4.5 °C. This was associated with collapsed chloroplast envelopes, disintegrated chloroplasts and very poor staining. Institute of Experimental Botany, ASCR, 2007 low temperature nationallicence photooxidation nationallicence photosynthesis nationallicence Zea mays nationallicence C4 : species that produce four-carbon acids as the primary initial CO2 fixation products nationallicence PN : net photosynthetic rate nationallicence PAR : photosynthetic active radiation nationallicence PPFD : photosynthetic photon flux density nationallicence TEM : transmission electron microscopy nationallicence Saropulos A. Department of Agricultural Botany, the University of Reading, RG6 2AU, Reading, UK aut Drennan D. Department of Agricultural Botany, the University of Reading, RG6 2AU, Reading, UK aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 690-698 0006-3134 51:4<690 2007 51 10535 https://doi.org/10.1007/s10535-007-0144-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0144-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saropulos A. Department of Agricultural Botany, the University of Reading, RG6 2AU, Reading, UK aut NATIONALLICENCE 700 1- Drennan D. Department of Agricultural Botany, the University of Reading, RG6 2AU, Reading, UK aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 690-698 0006-3134 51:4<690 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer