caa a22 4500 46316038X CHVBK 20180406164745.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0157-y doi (NATIONALLICENCE)springer-10.1007/s10535-007-0157-y In vitro propagation of Ophiorrhiza prostrata through somatic embryogenesis [Elektronische Daten] [K. Martin, A. Shahanaz Beegum, C.-L. Zhang, A. Slater, P. Madhusoodanan] In vitro propagation of an anticancerous drug synthesizing plant, Ophiorrhiza prostrata D. Don, was established through indirect somatic embryogenesis. Friable embryogenic calluses were initiated from O. prostrata leaf and internode explants on Murashige and Skoog (MS) media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) either alone or in combination with N6-benzyladenine (BA) or kinetin (KIN). Somatic embryos were developed after subculture of the friable calluses onto half strength MS media containing 0.45 or 2.26 µM 2,4-D alone or in combination with BA or KIN. Medium supplemented with 2.26 µM 2,4-D and 2.22 µM BA was optimal, supporting the production of a mean of 5.8 globular embryos. Subculture of globular embryo-bearing calluses on half strength MS medium without growth regulators produced the highest embryo frequency, and the majority of them developing to early torpedo stage. Somatic embryos underwent maturation and converted to plantlets at high frequency (90 %) on half strength MS medium supplemented with 0.44 µM BA. Somatic embryo-derived plantlets with well-developed roots were established in field conditions with a 90 % survival rate. Institute of Experimental Botany, ASCR, 2007 auxins nationallicence camptothecin-synthesizing plant nationallicence cytokinins nationallicence embryogenic callus nationallicence plantlets nationallicence BA : N6-benzyladenine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence KIN : kinetin nationallicence MS : Murashige and Skoog nationallicence NAA : α-naphthaleneacetic acid nationallicence PGRs : plant growth regulators nationallicence Martin K. Department of Biotechnology, University of Calicut, 673635, Kerala, India aut Shahanaz Beegum A. Department of Botany, University of Calicut, 673635, Kerala, India aut Zhang C.-L Norman Borlaug Institute for Plant Science Research, De Montfort University, LE1 9BH, Leicester, UK aut Slater A. Norman Borlaug Institute for Plant Science Research, De Montfort University, LE1 9BH, Leicester, UK aut Madhusoodanan P. Department of Botany, University of Calicut, 673635, Kerala, India aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 769-772 0006-3134 51:4<769 2007 51 10535 https://doi.org/10.1007/s10535-007-0157-y text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0157-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martin K. Department of Biotechnology, University of Calicut, 673635, Kerala, India aut NATIONALLICENCE 700 1- Shahanaz Beegum A. Department of Botany, University of Calicut, 673635, Kerala, India aut NATIONALLICENCE 700 1- Zhang C.-L Norman Borlaug Institute for Plant Science Research, De Montfort University, LE1 9BH, Leicester, UK aut NATIONALLICENCE 700 1- Slater A. Norman Borlaug Institute for Plant Science Research, De Montfort University, LE1 9BH, Leicester, UK aut NATIONALLICENCE 700 1- Madhusoodanan P. Department of Botany, University of Calicut, 673635, Kerala, India aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 769-772 0006-3134 51:4<769 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer