caa a22 4500 463160398 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0135-4 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0135-4 Direct somatic embryogenesis from leaves, cotyledons and hypocotyls of Hippophae rhamnoides [Elektronische Daten] [C. Liu, X. Xia, W. Yin, J. Zhou, H. Tang] Plant regeneration via direct somatic embryogenesis from cotyledons, hypocotyls and leaves in seabuckthorn (Hippophae rhamnoides L.) was achieved. The influences of basal media, carbon sources, plant growth regulators (PGRs) with different concentrations and combinations on embryogenesis capacity of explants were studied. The highest frequency of somatic embryos production and germination was obtained on Schenk and Hildebrandt medium (SH) supplemented with 1.0 mg dm−3 kinetin and 0.2, 0.5 mg dm−3 indole-3-acetic acid. Granulated sugar was the optimal carbon source. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse with a maximum survival rate of 55 %. Histological observation revealed that the somatic embryos were similar to those of zygotic embryos. Institute of Experimental Botany, ASCR, 2007 indole-3-butyric acid nationallicence in vitro morphogenesis nationallicence kinetin nationallicence plant regeneration nationallicence seabuckthorn nationallicence BA : 6-benzylaminopurine nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence FAA : formalin acetic acid ethanol nationallicence IAA : indole-3-acetic acid nationallicence IBA : indole-3-butyric acid nationallicence KIN : kinetin nationallicence MS medium : Murashige and Skoog medium nationallicence NAA : α-naphthaleneacetic acid nationallicence NN69 medium : Nitsch and Nitsch medium nationallicence PGR : plant growth regulator nationallicence SH medium : Schenk and Hildebrandt medium nationallicence WPM medium : Lloyd and McCown medium nationallicence Liu C. College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, P.R. China aut Xia X. College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, P.R. China aut Yin W. College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, P.R. China aut Zhou J. Phytopathology Key Laboratory, Yunnan Agricultural University, Kunming, 650201, Yunnan, P.R. China aut Tang H. College of Forest and Horticulture, Sichuan Agricultural University, Yaan, 625014, Sichuan, P.R. China aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 635-640 0006-3134 51:4<635 2007 51 10535 https://doi.org/10.1007/s10535-007-0135-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0135-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu C. College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, P.R. China aut NATIONALLICENCE 700 1- Xia X. College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, P.R. China aut NATIONALLICENCE 700 1- Yin W. College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, P.R. China aut NATIONALLICENCE 700 1- Zhou J. Phytopathology Key Laboratory, Yunnan Agricultural University, Kunming, 650201, Yunnan, P.R. China aut NATIONALLICENCE 700 1- Tang H. College of Forest and Horticulture, Sichuan Agricultural University, Yaan, 625014, Sichuan, P.R. China aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 635-640 0006-3134 51:4<635 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer