caa a22 4500 46316041X CHVBK 20180406164746.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0158-x doi (NATIONALLICENCE)springer-10.1007/s10535-007-0158-x The effect of sugars on niger embryogenesis and plant regeneration in anther culture [Elektronische Daten] [B. Hema, H. Murthy] The influence of different sugars (sucrose, maltose, glucose and fructose, 0.05-0.5 M) on embryogenesis and plant regeneration from cultured anthers of niger [Guizotia abyssinica (L. f.) Cass.] have been studied. Among the different sugars tested, 0.2 M sucrose was the best for embryo induction and plant regeneration. Maximum of 57 embryos per 60 anthers were induced on embryo induction medium [Gamborg's B5 medium supplemented with 10 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 2 µM kinetin (KIN)] containing 0.2 M sucrose. Embryo differentiation was achieved on B5 medium supplemented with 0.5 µM benzyladenine (BA) and 0.09 M sucrose. Embryo maturation was on B5 medium containing 10 µM abscisic acid (ABA) and 0.09 M sucrose. Embryo germination was achieved on B5 medium with 0.09 M sucrose. Embryos that were developed on B5 medium supplemented with 0.2 M sucrose showed highest frequency (68 %) of plant regeneration. Institute of Experimental Botany, ASCR, 2007 fructose nationallicence glucose nationallicence growth regulators nationallicence Guizotia abyssinica nationallicence haploids nationallicence maltose nationallicence sucrose nationallicence BA : N6-benzyladenine nationallicence B5 : Gamborg's medium nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence KIN : kinetin nationallicence ABA : abscisic acid nationallicence Hema B. Department of Botany, Karnatak University, 580003, Dharwad, India aut Murthy H. Department of Botany, Karnatak University, 580003, Dharwad, India aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 773-776 0006-3134 51:4<773 2007 51 10535 https://doi.org/10.1007/s10535-007-0158-x text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0158-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hema B. Department of Botany, Karnatak University, 580003, Dharwad, India aut NATIONALLICENCE 700 1- Murthy H. Department of Botany, Karnatak University, 580003, Dharwad, India aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 773-776 0006-3134 51:4<773 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer