caa a22 4500 463160428 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0136-3 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0136-3 Somatic embryogenesis and plant regeneration in Catharanthus roseus [Elektronische Daten] [A. Junaid, A. Mujib, M. Bhat, M. Sharma, J. Šamaj] Embryogenic callus in Catharanthus roseus was initiated from hypocotyl on Murashige and Skoog's (MS) medium supplemented with 1.0-2.0 mg dm−3 of 2,4-dichlorophenoxyacetic acid (2,4-D) or chlorophenoxyacetic acid (CPA). Calli from other sources were non-embryogenic. Numerous somatic embryos were induced from primary callus on MS medium suplemented with naphthalene acetic acid (NAA) within two weeks of culture. Embryo proliferation was much faster on medium supplemented with 6-benzylaminopurine (BAP). After transfer to medium with gibberellic acid (GA3, 1.0 mg dm− 3) mature green embryos were developed and germinated well into plantlets on MS liquid medium supplemented with 0.5 mg dm−3 BAP. Later, embryos with cotyledonary leaves were subjected to different auxins treatments for the development of roots. Before transfer ex vitro, plantlets were cultivated on half strength MS medium containing 3 % sucrose and 0.5 mg dm−3 BAP for additional 2 weeks. Additionally, the effect of liquid medium has been evaluated at different morphogenetic stages. Institute of Experimental Botany, ASCR, 2007 auxins nationallicence cytokinins nationallicence gibberellic acid nationallicence in vitro culture nationallicence ANOVA : analysis of variance nationallicence BAP : 6-benzylaminopurine nationallicence CPA : chlorophenoxyacetic acid nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence GA3 : gibberellic acid nationallicence IAA : indole-acetic acid nationallicence IBA : indole-butyric acid nationallicence KIN : kinetin nationallicence MS : Murashige and Skoog (1962) medium nationallicence NAA : naphthalene acetic acid nationallicence 2,4,5-T : 2,4,5-trihydroxy-butyrophenone nationallicence Junaid A. Cellular Differentiation and Molecular Genetics Section, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut Mujib A. Cellular Differentiation and Molecular Genetics Section, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut Bhat M. Cellular Differentiation and Molecular Genetics Section, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut Sharma M. Cellular Differentiation and Molecular Genetics Section, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut Šamaj J. Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 641-646 0006-3134 51:4<641 2007 51 10535 https://doi.org/10.1007/s10535-007-0136-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0136-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Junaid A. Cellular Differentiation and Molecular Genetics Section, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Mujib A. Cellular Differentiation and Molecular Genetics Section, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Bhat M. Cellular Differentiation and Molecular Genetics Section, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Sharma M. Cellular Differentiation and Molecular Genetics Section, Department of Botany, Faculty of Science, Hamdard University, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Šamaj J. Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 641-646 0006-3134 51:4<641 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer