caa a22 4500 463160452 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0133-6 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0133-6 The role of dehydrins in plant response to cold [Elektronische Daten] [K. Kosová, P. Vítámvás, I. Prášil] Dehydrins present a distinct biochemical group of late embryogenesis abundant (LEA) proteins characterised by the presence of a lysine-rich amino acid motif, the K-segment. They are highly hydrophilic, soluble upon boiling, and rich in glycine and polar amino acids. It is proposed that they can act as emulsifiers or chaperones in the cells, i.e., they protect proteins and membranes against unfavourable structural changes caused by dehydration. Cold usually precedes freezing in nature and induces many physiological and biochemical changes in the cells of freezing-tolerant plant species (cold-acclimation) that enable them to survive unfavourable conditions. It is demonstrated that the induction of dehydrin expression and their accumulation is an important part of this process in many dicotyledons (both herbaceous and woody species), and also in winter cultivars of cereals, especially wheat and barley. Some mechanisms which are proposed to be involved in regulation of dehydrin expression are discussed, i.e., endogenous content of abscisic acid, homologues of Arabidopsis C-repeat binding factor (CBF) transcriptional activators, the activity of vernalization genes and photoperiodic signals. Finally, we outline some new approaches emerging for the solution of the complex mechanisms involved in plant cold-acclimation, especially the methods of functional genomics that enable to observe simultaneously changes in the activity of many genes and proteins in a single sample. Institute of Experimental Botany, ASCR, 2007 abscisic acid nationallicence cereals nationallicence cold-acclimation nationallicence dicotyledons nationallicence frost resistance nationallicence K-segment nationallicence LEA D-11 proteins nationallicence low temperature stress nationallicence ABA : abscisic acid nationallicence ABRE : ABA-responsive element nationallicence bZIP : basic-domain leucine zipper nationallicence CaMV : cauliflower mosaic virus nationallicence CAT : catalase nationallicence CBF : C-repeat-binding factor nationallicence Cor : cold-regulated nationallicence CRT : C-repeat nationallicence Dhn : dehydrin nationallicence DRE : dehydration-responsive element nationallicence ELIPs : early light-inducible proteins nationallicence Erd : early response to drought nationallicence EST : expressed sequence tag nationallicence Fr gene : frost resistance gene nationallicence FT : frost tolerance nationallicence GUS : β-glucuronidase nationallicence LEA : late embryogenesis abundant nationallicence LD : long day nationallicence LDH : lactate dehydrogenase nationallicence LT : low temperature nationallicence LT50 : lethal temperature when 50 % samples die nationallicence Lti : low temperature-induced nationallicence LTRE : low temperature-responsive element nationallicence Mr : relative molecular mass nationallicence NLS : nuclear localisation sequence nationallicence PD50 : 50 % protein denaturation nationallicence pI : isoelectric point nationallicence Ppd : photoperiod nationallicence QTL : quantitative trait loci nationallicence Rab : response to ABA nationallicence RT-PCR : reverse transcriptase polymerase chain reaction nationallicence SD : short day nationallicence SDS-PAGE : sodium dodecyl sulphate polyacrylamide gel electrophoresis nationallicence UV CD : ultra-violet circular dichroism nationallicence Vrn : vernalization nationallicence Wcor : wheat cold-regulated nationallicence Wcs : wheat cold-specific nationallicence Wdhn : wheat dehydrin nationallicence WT : wild type nationallicence 2DE : two dimensional electrophoresis nationallicence 2D-DIGE : two dimensional difference gel electrophoresis nationallicence Kosová K. Research Institute of Crop Production, Drnovská 507, CZ-16106, Prague, Czech Republic aut Vítámvás P. Research Institute of Crop Production, Drnovská 507, CZ-16106, Prague, Czech Republic aut Prášil I. Research Institute of Crop Production, Drnovská 507, CZ-16106, Prague, Czech Republic aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 601-617 0006-3134 51:4<601 2007 51 10535 https://doi.org/10.1007/s10535-007-0133-6 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0133-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kosová K. Research Institute of Crop Production, Drnovská 507, CZ-16106, Prague, Czech Republic aut NATIONALLICENCE 700 1- Vítámvás P. Research Institute of Crop Production, Drnovská 507, CZ-16106, Prague, Czech Republic aut NATIONALLICENCE 700 1- Prášil I. Research Institute of Crop Production, Drnovská 507, CZ-16106, Prague, Czech Republic aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 601-617 0006-3134 51:4<601 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer