caa a22 4500 463160460 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0152-3 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0152-3 Transgenic tobacco plants containing Bt and GNA genes [Elektronische Daten] [H. Zhang, X. Liu, L. Wei, L. Zhou, Y. Yang] A new plant expression vector (pBSbtCry1Ac-GNA) containing two insect resistant genes, a synthetic chimeric gene SbtCry1Ac encoding the insecticidal protein CrylAc and a gene GNA encoding snowdrop lectin (Galanthus nivalis agglutinin) was constructed. Transgenic tobacco plants containing these two genes were obtained through Agrobacterium-mediated transformation of tobacco leaf discs. Results from PCR detection and genomic DNA Southern blot analysis indicated that both SbtCrylAc gene and GNA gene were integrated into the genome of these plants. Results of Western blot analysis indicated that these two proteins were expressed in the analyzed plants. Bioassays of Myzus persicae and Helicoverpa assulta on detached leaves of transformed tobacco plants were carried out. The average aphid inhibition rate of these plants tested at 12 d post-infestation was 71.9 %. The average H. assulta mortality of these plants tested at 6 d post-infestation was up to 89.8 %. The kanamycin resistance of the T1 progeny of these transgenic plants was analyzed and a typical 3:1 segregation was observed. Institute of Experimental Botany, ASCR, 2007 agglutinin nationallicence insect-resistant nationallicence SbtcrylAc nationallicence Bt : Bacillus thurigiensis nationallicence GNA : Galanthus nivalis agglutinin nationallicence MS : Murashige and Skoog nationallicence PCR : polymerase chain reaction nationallicence SDS-PAGE : sodium dodecyl sulphate-polyacrylamide gel electrophoresis nationallicence Zhang H. Southwest Forestry College, White Dragon Temple, 650224, Kunming, Yunnan Province, P.R. China aut Liu X. Southwest Forestry College, White Dragon Temple, 650224, Kunming, Yunnan Province, P.R. China aut Wei L. Institute of Microbiology, Chinese Academy of Science, 100080, Beijing, P.R. China aut Zhou L. Institute of Microbiology, Chinese Academy of Science, 100080, Beijing, P.R. China aut Yang Y. Southwest Forestry College, White Dragon Temple, 650224, Kunming, Yunnan Province, P.R. China aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 746-748 0006-3134 51:4<746 2007 51 10535 https://doi.org/10.1007/s10535-007-0152-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0152-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang H. Southwest Forestry College, White Dragon Temple, 650224, Kunming, Yunnan Province, P.R. China aut NATIONALLICENCE 700 1- Liu X. Southwest Forestry College, White Dragon Temple, 650224, Kunming, Yunnan Province, P.R. China aut NATIONALLICENCE 700 1- Wei L. Institute of Microbiology, Chinese Academy of Science, 100080, Beijing, P.R. China aut NATIONALLICENCE 700 1- Zhou L. Institute of Microbiology, Chinese Academy of Science, 100080, Beijing, P.R. China aut NATIONALLICENCE 700 1- Yang Y. Southwest Forestry College, White Dragon Temple, 650224, Kunming, Yunnan Province, P.R. China aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 746-748 0006-3134 51:4<746 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer