caa a22 4500 463160487 CHVBK 20180406164746.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10535-007-0161-2 doi (NATIONALLICENCE)springer-10.1007/s10535-007-0161-2 Rapid micropropagation of Ocimum basilicum using shoot tip explants pre-cultured in thidiazuron supplemented liquid medium [Elektronische Daten] [I. Siddique, M. Anis] An efficient protocol has been developed for rapid micropropagation of Ocimum basilicum. Multiple shoots were induced by culturing shoot tip explants excised from mature plants on a liquid Murashige and Skoog (MS) medium supplemented with 5-100 µM of thidiazuron (TDZ) for different treatment duration (4, 8, 12 and 16 d). The optimal level of TDZ supplementation to the culture medium was 50 µM for 8 d induction period followed by subculturing in MS medium devoid of TDZ as it produced maximum regeneration frequency (78 %), mean number of shoots (11.6 ± 1.16) and shoot length (4.8 ± 0.43 cm) per explant. A culture period longer than 8 d with TDZ resulted in the formation of fasciated or distorted shoots. The regenerated shoots rooted best on MS medium containing 1.0 µM indole-3-butyric acid (IBA). The micropropagated shoots with well developed roots were successfully established in pots containing garden soil and grown in greenhouse with 95 % survival rate. The regenerated plants were morphologically uniform and exhibited similar growth characteristics and vegetative morphology to the donor plants. Institute of Experimental Botany, ASCR, 2007 growth characteristics nationallicence indole-3-butyric acid nationallicence shoot multiplication nationallicence Soilrite nationallicence sweet basil nationallicence IBA : indole-3-butyric acid nationallicence MS : Murashige and Skoog medium nationallicence PGR : plant growth regulator nationallicence PPFD : photosynthetic photon flux density nationallicence TDZ : thidiazuron nationallicence Siddique I. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, 202002, Aligarh, India aut Anis M. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, 202002, Aligarh, India aut Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 787-790 0006-3134 51:4<787 2007 51 10535 https://doi.org/10.1007/s10535-007-0161-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10535-007-0161-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Siddique I. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, 202002, Aligarh, India aut NATIONALLICENCE 700 1- Anis M. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, 202002, Aligarh, India aut NATIONALLICENCE 773 0- Biologia Plantarum Springer Netherlands 51/4(2007-12-01), 787-790 0006-3134 51:4<787 2007 51 10535 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer